Experiment: dextran, Mw ~200,000; filtered 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2650 and BT2651 are separated by 57 nucleotides BT2651 and BT2652 are separated by 347 nucleotides BT2652 and BT2653 are separated by 10 nucleotides BT2653 and BT2654 are separated by 691 nucleotides
BT2650: BT2650 - conserved hypothetical protein (NCBI ptt file), at 3,302,647 to 3,303,348
BT2650
BT2651: BT2651 - conserved hypothetical protein (NCBI ptt file), at 3,303,406 to 3,303,699
BT2651
BT2652: BT2652 - conserved hypothetical protein (NCBI ptt file), at 3,304,047 to 3,304,334
BT2652
BT2653: BT2653 - hypothetical protein (NCBI ptt file), at 3,304,345 to 3,304,638
BT2653
BT2654: BT2654 - transposase (NCBI ptt file), at 3,305,330 to 3,306,541
BT2654
Position (kb)
3304
3305 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3303.123 kb on + strand, within BT2650 at 3303.224 kb on + strand, within BT2650 at 3303.225 kb on - strand, within BT2650 at 3303.226 kb on + strand, within BT2650 at 3303.227 kb on - strand, within BT2650 at 3303.227 kb on - strand, within BT2650 at 3303.229 kb on - strand, within BT2650 at 3303.229 kb on - strand, within BT2650 at 3303.229 kb on - strand, within BT2650 at 3303.391 kb on + strand at 3303.394 kb on - strand at 3303.488 kb on + strand, within BT2651 at 3303.654 kb on + strand, within BT2651 at 3303.654 kb on + strand, within BT2651 at 3303.684 kb on + strand at 3303.684 kb on + strand at 3303.685 kb on - strand at 3303.688 kb on + strand at 3303.688 kb on + strand at 3303.688 kb on + strand at 3303.688 kb on + strand at 3303.688 kb on + strand at 3303.689 kb on - strand at 3303.693 kb on - strand at 3303.693 kb on - strand at 3303.738 kb on + strand at 3303.756 kb on + strand at 3303.764 kb on + strand at 3303.766 kb on + strand at 3303.766 kb on + strand at 3303.769 kb on + strand at 3303.769 kb on + strand at 3303.769 kb on + strand at 3303.848 kb on + strand at 3303.899 kb on + strand at 3304.015 kb on + strand at 3304.138 kb on + strand, within BT2652 at 3304.190 kb on + strand, within BT2652 at 3304.199 kb on + strand, within BT2652 at 3304.299 kb on + strand, within BT2652 at 3304.453 kb on - strand, within BT2653 at 3304.471 kb on + strand, within BT2653 at 3304.472 kb on - strand, within BT2653 at 3304.508 kb on + strand, within BT2653 at 3304.556 kb on - strand, within BT2653 at 3304.577 kb on + strand, within BT2653 at 3304.582 kb on + strand, within BT2653 at 3304.583 kb on - strand, within BT2653 at 3304.583 kb on - strand, within BT2653 at 3304.583 kb on - strand, within BT2653 at 3304.596 kb on + strand, within BT2653 at 3304.596 kb on + strand, within BT2653 at 3304.597 kb on - strand, within BT2653 at 3304.637 kb on - strand at 3304.651 kb on + strand at 3304.652 kb on - strand at 3304.696 kb on + strand at 3304.697 kb on - strand at 3304.698 kb on + strand at 3304.698 kb on + strand at 3304.698 kb on + strand at 3304.699 kb on - strand at 3304.699 kb on - strand at 3304.700 kb on + strand at 3304.700 kb on + strand at 3304.701 kb on - strand at 3304.720 kb on - strand at 3304.732 kb on + strand at 3304.753 kb on - strand at 3304.832 kb on + strand at 3304.972 kb on + strand at 3304.972 kb on + strand at 3304.975 kb on + strand at 3304.975 kb on + strand at 3304.975 kb on + strand at 3305.026 kb on + strand at 3305.027 kb on - strand at 3305.053 kb on + strand at 3305.057 kb on + strand at 3305.057 kb on + strand at 3305.058 kb on - strand at 3305.086 kb on - strand at 3305.105 kb on + strand at 3305.115 kb on + strand at 3305.150 kb on + strand at 3305.177 kb on + strand at 3305.178 kb on - strand at 3305.186 kb on + strand at 3305.187 kb on - strand at 3305.205 kb on + strand at 3305.205 kb on + strand at 3305.287 kb on + strand at 3305.287 kb on + strand at 3305.288 kb on - strand at 3305.297 kb on + strand at 3305.304 kb on - strand at 3305.308 kb on + strand at 3305.309 kb on - strand at 3305.325 kb on + strand at 3305.326 kb on - strand at 3305.330 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction dextran, Mw ~200,000; filtered 2.5 mg/ml (C) remove 3,303,123 + BT2650 0.68 -1.5 3,303,224 + BT2650 0.82 -2.2 3,303,225 - BT2650 0.82 -1.8 3,303,226 + BT2650 0.82 -0.8 3,303,227 - BT2650 0.83 -1.0 3,303,227 - BT2650 0.83 +0.0 3,303,229 - BT2650 0.83 +1.0 3,303,229 - BT2650 0.83 -1.6 3,303,229 - BT2650 0.83 +0.0 3,303,391 + -1.7 3,303,394 - -2.8 3,303,488 + BT2651 0.28 -0.6 3,303,654 + BT2651 0.84 -2.9 3,303,654 + BT2651 0.84 -1.9 3,303,684 + -1.2 3,303,684 + -1.9 3,303,685 - +0.0 3,303,688 + +0.0 3,303,688 + -0.6 3,303,688 + +1.0 3,303,688 + -1.1 3,303,688 + -0.6 3,303,689 - +0.0 3,303,693 - -0.8 3,303,693 - +0.0 3,303,738 + -0.8 3,303,756 + -1.1 3,303,764 + -1.2 3,303,766 + -1.3 3,303,766 + -1.6 3,303,769 + -0.9 3,303,769 + +0.0 3,303,769 + -1.0 3,303,848 + -1.5 3,303,899 + -1.0 3,304,015 + -2.7 3,304,138 + BT2652 0.32 -2.0 3,304,190 + BT2652 0.50 -2.2 3,304,199 + BT2652 0.53 -1.7 3,304,299 + BT2652 0.88 -1.6 3,304,453 - BT2653 0.37 -2.0 3,304,471 + BT2653 0.43 -2.5 3,304,472 - BT2653 0.43 +2.0 3,304,508 + BT2653 0.55 -1.9 3,304,556 - BT2653 0.72 -1.4 3,304,577 + BT2653 0.79 -0.7 3,304,582 + BT2653 0.81 -0.9 3,304,583 - BT2653 0.81 -0.9 3,304,583 - BT2653 0.81 -1.6 3,304,583 - BT2653 0.81 -1.2 3,304,596 + BT2653 0.85 -1.6 3,304,596 + BT2653 0.85 -0.7 3,304,597 - BT2653 0.86 -1.0 3,304,637 - -1.0 3,304,651 + +2.0 3,304,652 - +0.0 3,304,696 + +0.0 3,304,697 - -1.0 3,304,698 + +0.0 3,304,698 + -2.1 3,304,698 + +0.8 3,304,699 - -0.5 3,304,699 - +0.0 3,304,700 + -1.6 3,304,700 + -0.1 3,304,701 - -2.3 3,304,720 - +1.6 3,304,732 + -1.0 3,304,753 - -2.6 3,304,832 + -1.3 3,304,972 + +0.0 3,304,972 + -0.5 3,304,975 + -1.4 3,304,975 + -0.6 3,304,975 + -0.7 3,305,026 + -1.2 3,305,027 - +0.0 3,305,053 + +1.6 3,305,057 + +1.6 3,305,057 + -1.0 3,305,058 - -1.0 3,305,086 - -0.8 3,305,105 + -1.8 3,305,115 + -0.4 3,305,150 + -1.0 3,305,177 + +1.0 3,305,178 - -1.0 3,305,186 + -0.6 3,305,187 - -2.6 3,305,205 + +0.0 3,305,205 + -2.0 3,305,287 + -1.9 3,305,287 + -1.3 3,305,288 - -2.2 3,305,297 + -1.3 3,305,304 - -1.5 3,305,308 + +0.0 3,305,309 - -0.0 3,305,325 + +0.0 3,305,326 - -1.6 3,305,330 - -1.6
Or see this region's nucleotide sequence