Experiment: dextran, Mw ~200,000; filtered 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0400 and BT0401 overlap by 4 nucleotides BT0401 and BT0402 are separated by 251 nucleotides BT0402 and BT0403 are separated by 7 nucleotides BT0403 and BT0404 are separated by 258 nucleotides
BT0400: BT0400 - putative tyrosine-protein kinase in capsular polysaccharide biosynthesis region (NCBI ptt file), at 488,578 to 489,867
BT0400
BT0401: BT0401 - hypothetical protein (NCBI ptt file), at 489,864 to 490,082
BT0401
BT0402: BT0402 - hypothetical protein (NCBI ptt file), at 490,334 to 490,843
BT0402
BT0403: BT0403 - hypothetical protein (NCBI ptt file), at 490,851 to 491,348
BT0403
BT0404: BT0404 - hypothetical protein (NCBI ptt file), at 491,607 to 493,934
BT0404
Position (kb) 490
491 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 489.723 kb on + strand, within BT0400 at 489.787 kb on + strand at 490.026 kb on + strand, within BT0401 at 490.070 kb on - strand at 490.103 kb on - strand at 490.146 kb on + strand at 490.187 kb on + strand at 490.266 kb on - strand at 490.318 kb on + strand at 490.332 kb on + strand at 490.360 kb on - strand at 490.474 kb on + strand, within BT0402 at 490.474 kb on + strand, within BT0402 at 490.614 kb on - strand, within BT0402 at 490.667 kb on - strand, within BT0402 at 490.667 kb on - strand, within BT0402 at 490.714 kb on - strand, within BT0402 at 490.968 kb on - strand, within BT0403 at 490.968 kb on - strand, within BT0403 at 490.970 kb on - strand, within BT0403 at 491.386 kb on + strand at 491.413 kb on + strand at 491.598 kb on - strand at 491.604 kb on - strand at 491.638 kb on - strand at 491.638 kb on - strand at 491.774 kb on + strand at 491.829 kb on + strand at 491.838 kb on - strand at 491.838 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction dextran, Mw ~200,000; filtered 2.5 mg/ml (C) remove 489,723 + BT0400 0.89 +0.9 489,787 + -0.7 490,026 + BT0401 0.74 -1.7 490,070 - -0.4 490,103 - -0.1 490,146 + +0.9 490,187 + -1.4 490,266 - -0.7 490,318 + -1.1 490,332 + -1.7 490,360 - +2.1 490,474 + BT0402 0.27 -1.9 490,474 + BT0402 0.27 -1.7 490,614 - BT0402 0.55 -1.7 490,667 - BT0402 0.65 -1.7 490,667 - BT0402 0.65 -0.1 490,714 - BT0402 0.75 -2.9 490,968 - BT0403 0.23 +0.9 490,968 - BT0403 0.23 -0.1 490,970 - BT0403 0.24 -0.1 491,386 + -1.4 491,413 + -1.9 491,598 - -0.1 491,604 - +0.7 491,638 - -1.3 491,638 - -0.1 491,774 + -0.1 491,829 + -2.9 491,838 - -1.2 491,838 - -1.4
Or see this region's nucleotide sequence