Experiment: dextran, Mw ~200,000; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2212 and BT2213 are separated by 11 nucleotides BT2213 and BT2214 overlap by 4 nucleotides BT2214 and BT2215 are separated by 20 nucleotides
BT2212: BT2212 - magnesium chelatase, subunit I, putative ATPase (NCBI ptt file), at 2,780,158 to 2,781,132
BT2212
BT2213: BT2213 - hypothetical protein (NCBI ptt file), at 2,781,144 to 2,782,418
BT2213
BT2214: BT2214 - hypothetical protein (NCBI ptt file), at 2,782,415 to 2,783,029
BT2214
BT2215: BT2215 - hypothetical protein (NCBI ptt file), at 2,783,050 to 2,783,982
BT2215
Position (kb)
2781
2782
2783 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2780.379 kb on - strand, within BT2212 at 2780.380 kb on + strand, within BT2212 at 2780.424 kb on - strand, within BT2212 at 2780.436 kb on + strand, within BT2212 at 2780.436 kb on + strand, within BT2212 at 2780.443 kb on + strand, within BT2212 at 2780.524 kb on + strand, within BT2212 at 2780.538 kb on + strand, within BT2212 at 2780.538 kb on + strand, within BT2212 at 2780.582 kb on + strand, within BT2212 at 2780.676 kb on + strand, within BT2212 at 2780.681 kb on + strand, within BT2212 at 2780.682 kb on - strand, within BT2212 at 2780.698 kb on - strand, within BT2212 at 2780.719 kb on + strand, within BT2212 at 2780.720 kb on - strand, within BT2212 at 2780.795 kb on - strand, within BT2212 at 2780.848 kb on - strand, within BT2212 at 2780.987 kb on - strand, within BT2212 at 2781.132 kb on + strand at 2781.132 kb on + strand at 2781.136 kb on + strand at 2781.146 kb on - strand at 2781.146 kb on - strand at 2781.180 kb on - strand at 2781.367 kb on + strand, within BT2213 at 2781.438 kb on - strand, within BT2213 at 2781.503 kb on + strand, within BT2213 at 2781.503 kb on + strand, within BT2213 at 2781.541 kb on + strand, within BT2213 at 2781.542 kb on - strand, within BT2213 at 2781.617 kb on + strand, within BT2213 at 2781.617 kb on + strand, within BT2213 at 2781.619 kb on + strand at 2781.676 kb on + strand, within BT2213 at 2781.787 kb on + strand, within BT2213 at 2781.788 kb on - strand, within BT2213 at 2781.788 kb on - strand, within BT2213 at 2781.968 kb on - strand, within BT2213 at 2782.034 kb on - strand, within BT2213 at 2782.055 kb on + strand, within BT2213 at 2782.055 kb on + strand, within BT2213 at 2782.100 kb on + strand, within BT2213 at 2782.100 kb on + strand, within BT2213 at 2782.101 kb on - strand, within BT2213 at 2782.101 kb on - strand, within BT2213 at 2782.101 kb on - strand, within BT2213 at 2782.134 kb on + strand, within BT2213 at 2782.134 kb on + strand, within BT2213 at 2782.135 kb on - strand, within BT2213 at 2782.135 kb on - strand, within BT2213 at 2782.184 kb on + strand, within BT2213 at 2782.238 kb on + strand, within BT2213 at 2782.239 kb on - strand, within BT2213 at 2782.330 kb on - strand at 2782.356 kb on + strand at 2782.560 kb on + strand, within BT2214 at 2782.576 kb on + strand, within BT2214 at 2782.577 kb on - strand, within BT2214 at 2782.578 kb on + strand, within BT2214 at 2782.742 kb on - strand, within BT2214 at 2782.763 kb on + strand, within BT2214 at 2782.764 kb on - strand, within BT2214 at 2782.764 kb on - strand, within BT2214 at 2782.781 kb on - strand at 2782.813 kb on + strand, within BT2214 at 2782.814 kb on - strand, within BT2214 at 2782.826 kb on + strand, within BT2214 at 2782.827 kb on - strand, within BT2214 at 2782.827 kb on - strand, within BT2214 at 2782.827 kb on - strand, within BT2214 at 2782.830 kb on - strand, within BT2214 at 2782.832 kb on + strand, within BT2214 at 2782.833 kb on - strand, within BT2214 at 2782.934 kb on + strand, within BT2214 at 2782.950 kb on + strand, within BT2214 at 2783.031 kb on + strand at 2783.041 kb on + strand at 2783.045 kb on + strand at 2783.045 kb on + strand at 2783.045 kb on + strand at 2783.051 kb on + strand at 2783.067 kb on + strand at 2783.089 kb on + strand at 2783.094 kb on + strand at 2783.098 kb on + strand at 2783.099 kb on - strand at 2783.179 kb on + strand, within BT2215 at 2783.180 kb on - strand, within BT2215 at 2783.185 kb on - strand, within BT2215 at 2783.209 kb on - strand, within BT2215 at 2783.211 kb on - strand, within BT2215 at 2783.249 kb on - strand, within BT2215 at 2783.261 kb on + strand, within BT2215 at 2783.283 kb on - strand, within BT2215 at 2783.296 kb on - strand, within BT2215 at 2783.312 kb on - strand, within BT2215 at 2783.316 kb on + strand, within BT2215 at 2783.316 kb on + strand, within BT2215 at 2783.316 kb on + strand, within BT2215 at 2783.316 kb on + strand, within BT2215 at 2783.316 kb on + strand, within BT2215 at 2783.317 kb on - strand, within BT2215 at 2783.322 kb on + strand, within BT2215 at 2783.323 kb on - strand, within BT2215 at 2783.323 kb on - strand, within BT2215 at 2783.323 kb on - strand, within BT2215 at 2783.409 kb on + strand, within BT2215
Per-strain Table
Position Strand Gene LocusTag Fraction dextran, Mw ~200,000; autoclaved 2.5 mg/ml (C) remove 2,780,379 - BT2212 0.23 -1.3 2,780,380 + BT2212 0.23 -2.2 2,780,424 - BT2212 0.27 -1.0 2,780,436 + BT2212 0.29 +1.0 2,780,436 + BT2212 0.29 -0.3 2,780,443 + BT2212 0.29 -0.6 2,780,524 + BT2212 0.38 +0.0 2,780,538 + BT2212 0.39 -0.7 2,780,538 + BT2212 0.39 -1.0 2,780,582 + BT2212 0.43 -1.0 2,780,676 + BT2212 0.53 -2.8 2,780,681 + BT2212 0.54 -1.0 2,780,682 - BT2212 0.54 -1.6 2,780,698 - BT2212 0.55 -0.8 2,780,719 + BT2212 0.58 -3.0 2,780,720 - BT2212 0.58 -0.7 2,780,795 - BT2212 0.65 +0.0 2,780,848 - BT2212 0.71 +0.6 2,780,987 - BT2212 0.85 -1.8 2,781,132 + -0.6 2,781,132 + +2.0 2,781,136 + -0.9 2,781,146 - -0.6 2,781,146 - -2.0 2,781,180 - +0.0 2,781,367 + BT2213 0.17 -3.1 2,781,438 - BT2213 0.23 -1.2 2,781,503 + BT2213 0.28 +0.7 2,781,503 + BT2213 0.28 -0.2 2,781,541 + BT2213 0.31 -0.4 2,781,542 - BT2213 0.31 -0.8 2,781,617 + BT2213 0.37 -0.4 2,781,617 + BT2213 0.37 +1.0 2,781,619 + +0.0 2,781,676 + BT2213 0.42 -1.1 2,781,787 + BT2213 0.50 +0.0 2,781,788 - BT2213 0.51 -0.3 2,781,788 - BT2213 0.51 +0.2 2,781,968 - BT2213 0.65 +0.0 2,782,034 - BT2213 0.70 +0.0 2,782,055 + BT2213 0.71 -1.0 2,782,055 + BT2213 0.71 -0.6 2,782,100 + BT2213 0.75 -0.7 2,782,100 + BT2213 0.75 -1.3 2,782,101 - BT2213 0.75 -0.8 2,782,101 - BT2213 0.75 -1.0 2,782,101 - BT2213 0.75 -1.6 2,782,134 + BT2213 0.78 -2.0 2,782,134 + BT2213 0.78 -0.9 2,782,135 - BT2213 0.78 -0.7 2,782,135 - BT2213 0.78 -1.0 2,782,184 + BT2213 0.82 +1.0 2,782,238 + BT2213 0.86 -0.3 2,782,239 - BT2213 0.86 +0.0 2,782,330 - -1.6 2,782,356 + +0.0 2,782,560 + BT2214 0.24 -2.1 2,782,576 + BT2214 0.26 +0.0 2,782,577 - BT2214 0.26 +0.8 2,782,578 + BT2214 0.27 -1.8 2,782,742 - BT2214 0.53 -1.0 2,782,763 + BT2214 0.57 -0.9 2,782,764 - BT2214 0.57 -1.2 2,782,764 - BT2214 0.57 +1.0 2,782,781 - +1.0 2,782,813 + BT2214 0.65 -0.7 2,782,814 - BT2214 0.65 +1.6 2,782,826 + BT2214 0.67 -3.0 2,782,827 - BT2214 0.67 +0.0 2,782,827 - BT2214 0.67 -2.0 2,782,827 - BT2214 0.67 -1.3 2,782,830 - BT2214 0.67 -1.6 2,782,832 + BT2214 0.68 -1.2 2,782,833 - BT2214 0.68 -0.5 2,782,934 + BT2214 0.84 -1.6 2,782,950 + BT2214 0.87 -1.0 2,783,031 + -1.4 2,783,041 + +0.0 2,783,045 + -0.8 2,783,045 + -1.5 2,783,045 + -1.6 2,783,051 + -1.0 2,783,067 + -2.2 2,783,089 + -1.0 2,783,094 + -0.9 2,783,098 + +1.4 2,783,099 - -2.0 2,783,179 + BT2215 0.14 -3.3 2,783,180 - BT2215 0.14 -0.1 2,783,185 - BT2215 0.14 -1.3 2,783,209 - BT2215 0.17 +0.0 2,783,211 - BT2215 0.17 -1.0 2,783,249 - BT2215 0.21 -2.0 2,783,261 + BT2215 0.23 +0.0 2,783,283 - BT2215 0.25 -2.0 2,783,296 - BT2215 0.26 +0.6 2,783,312 - BT2215 0.28 -1.0 2,783,316 + BT2215 0.29 -1.7 2,783,316 + BT2215 0.29 -1.0 2,783,316 + BT2215 0.29 -0.8 2,783,316 + BT2215 0.29 -1.6 2,783,316 + BT2215 0.29 -0.4 2,783,317 - BT2215 0.29 +0.0 2,783,322 + BT2215 0.29 +1.6 2,783,323 - BT2215 0.29 +1.6 2,783,323 - BT2215 0.29 -1.2 2,783,323 - BT2215 0.29 +1.0 2,783,409 + BT2215 0.38 +0.0
Or see this region's nucleotide sequence