Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35705 and BPHYT_RS35710 are separated by 65 nucleotides BPHYT_RS35710 and BPHYT_RS35715 are separated by 310 nucleotides BPHYT_RS35715 and BPHYT_RS35720 are separated by 87 nucleotides BPHYT_RS35720 and BPHYT_RS35725 are separated by 20 nucleotides
BPHYT_RS35705: BPHYT_RS35705 - FAD-dependent oxidoreductase, at 3,601,865 to 3,603,574
_RS35705
BPHYT_RS35710: BPHYT_RS35710 - hypothetical protein, at 3,603,640 to 3,603,876
_RS35710
BPHYT_RS35715: BPHYT_RS35715 - hypothetical protein, at 3,604,187 to 3,604,663
_RS35715
BPHYT_RS35720: BPHYT_RS35720 - phosphopantetheinyl transferase, at 3,604,751 to 3,605,446
_RS35720
BPHYT_RS35725: BPHYT_RS35725 - hypothetical protein, at 3,605,467 to 3,606,312
_RS35725
Position (kb)
3604
3605 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3603.977 kb on + strand at 3604.027 kb on - strand at 3604.053 kb on + strand at 3604.205 kb on - strand at 3604.718 kb on + strand at 3604.755 kb on - strand at 3604.857 kb on + strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3604.858 kb on - strand, within BPHYT_RS35720 at 3605.055 kb on + strand, within BPHYT_RS35720 at 3605.055 kb on + strand, within BPHYT_RS35720 at 3605.419 kb on - strand at 3605.419 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,603,977 + -1.3 3,604,027 - -0.9 3,604,053 + +0.8 3,604,205 - +1.1 3,604,718 + -0.4 3,604,755 - +0.4 3,604,857 + BPHYT_RS35720 0.15 -0.1 3,604,858 - BPHYT_RS35720 0.15 +0.9 3,604,858 - BPHYT_RS35720 0.15 -2.8 3,604,858 - BPHYT_RS35720 0.15 -0.2 3,604,858 - BPHYT_RS35720 0.15 +0.2 3,605,055 + BPHYT_RS35720 0.44 -0.5 3,605,055 + BPHYT_RS35720 0.44 -0.1 3,605,419 - +0.1 3,605,419 - +1.5
Or see this region's nucleotide sequence