Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35655 and BPHYT_RS35660 are separated by 62 nucleotides BPHYT_RS35660 and BPHYT_RS35665 overlap by 4 nucleotides BPHYT_RS35665 and BPHYT_RS35670 are separated by 145 nucleotides
BPHYT_RS35655: BPHYT_RS35655 - hypothetical protein, at 3,592,015 to 3,592,308
_RS35655
BPHYT_RS35660: BPHYT_RS35660 - membrane protein, at 3,592,371 to 3,593,222
_RS35660
BPHYT_RS35665: BPHYT_RS35665 - ABC transporter permease, at 3,593,219 to 3,594,109
_RS35665
BPHYT_RS35670: BPHYT_RS35670 - bicyclomycin resistance protein, at 3,594,255 to 3,595,487
_RS35670
Position (kb) 3593
3594
3595 Strain fitness (log2 ratio) -1
0
1 at 3592.222 kb on + strand, within BPHYT_RS35655 at 3592.238 kb on - strand, within BPHYT_RS35655 at 3592.372 kb on + strand at 3592.373 kb on - strand at 3592.408 kb on + strand at 3592.428 kb on + strand at 3592.493 kb on - strand, within BPHYT_RS35660 at 3592.559 kb on + strand, within BPHYT_RS35660 at 3592.817 kb on - strand, within BPHYT_RS35660 at 3593.001 kb on - strand, within BPHYT_RS35660 at 3593.001 kb on - strand, within BPHYT_RS35660 at 3593.154 kb on + strand at 3593.350 kb on - strand, within BPHYT_RS35665 at 3593.355 kb on + strand, within BPHYT_RS35665 at 3593.356 kb on - strand, within BPHYT_RS35665 at 3593.434 kb on - strand, within BPHYT_RS35665 at 3593.811 kb on + strand, within BPHYT_RS35665 at 3594.123 kb on + strand at 3594.140 kb on + strand at 3594.178 kb on + strand at 3594.605 kb on - strand, within BPHYT_RS35670 at 3594.899 kb on - strand, within BPHYT_RS35670 at 3595.042 kb on + strand, within BPHYT_RS35670 at 3595.043 kb on - strand, within BPHYT_RS35670 at 3595.045 kb on + strand, within BPHYT_RS35670
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,592,222 + BPHYT_RS35655 0.70 +1.0 3,592,238 - BPHYT_RS35655 0.76 +1.2 3,592,372 + -0.4 3,592,373 - +0.3 3,592,408 + -0.4 3,592,428 + +0.9 3,592,493 - BPHYT_RS35660 0.14 +0.1 3,592,559 + BPHYT_RS35660 0.22 +0.4 3,592,817 - BPHYT_RS35660 0.52 +0.2 3,593,001 - BPHYT_RS35660 0.74 +0.0 3,593,001 - BPHYT_RS35660 0.74 -1.4 3,593,154 + +0.9 3,593,350 - BPHYT_RS35665 0.15 -0.1 3,593,355 + BPHYT_RS35665 0.15 +0.7 3,593,356 - BPHYT_RS35665 0.15 -0.2 3,593,434 - BPHYT_RS35665 0.24 -1.2 3,593,811 + BPHYT_RS35665 0.66 -0.8 3,594,123 + +1.1 3,594,140 + +1.1 3,594,178 + -0.3 3,594,605 - BPHYT_RS35670 0.28 +0.0 3,594,899 - BPHYT_RS35670 0.52 -1.3 3,595,042 + BPHYT_RS35670 0.64 +0.4 3,595,043 - BPHYT_RS35670 0.64 -1.0 3,595,045 + BPHYT_RS35670 0.64 +0.2
Or see this region's nucleotide sequence