Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35625 and BPHYT_RS35630 are separated by 272 nucleotides BPHYT_RS35630 and BPHYT_RS35635 are separated by 200 nucleotides BPHYT_RS35635 and BPHYT_RS35640 are separated by 145 nucleotides
BPHYT_RS35625: BPHYT_RS35625 - ser/threonine protein phosphatase, at 3,585,436 to 3,587,127
_RS35625
BPHYT_RS35630: BPHYT_RS35630 - membrane protein, at 3,587,400 to 3,587,840
_RS35630
BPHYT_RS35635: BPHYT_RS35635 - nucleoside 2-deoxyribosyltransferase, at 3,588,041 to 3,588,625
_RS35635
BPHYT_RS35640: BPHYT_RS35640 - hypothetical protein, at 3,588,771 to 3,589,217
_RS35640
Position (kb)
3588
3589 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3587.897 kb on + strand at 3588.053 kb on + strand at 3588.054 kb on - strand at 3588.256 kb on + strand, within BPHYT_RS35635 at 3588.261 kb on - strand, within BPHYT_RS35635 at 3588.450 kb on + strand, within BPHYT_RS35635 at 3588.742 kb on + strand at 3588.743 kb on - strand at 3588.773 kb on - strand at 3588.923 kb on - strand, within BPHYT_RS35640 at 3588.923 kb on - strand, within BPHYT_RS35640 at 3588.983 kb on - strand, within BPHYT_RS35640 at 3589.021 kb on - strand, within BPHYT_RS35640 at 3589.081 kb on - strand, within BPHYT_RS35640 at 3589.103 kb on - strand, within BPHYT_RS35640 at 3589.103 kb on - strand, within BPHYT_RS35640 at 3589.206 kb on - strand at 3589.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,587,897 + -0.0 3,588,053 + +0.2 3,588,054 - -1.8 3,588,256 + BPHYT_RS35635 0.37 +0.3 3,588,261 - BPHYT_RS35635 0.38 -0.3 3,588,450 + BPHYT_RS35635 0.70 +0.1 3,588,742 + +0.2 3,588,743 - +0.4 3,588,773 - +0.8 3,588,923 - BPHYT_RS35640 0.34 +0.5 3,588,923 - BPHYT_RS35640 0.34 +0.7 3,588,983 - BPHYT_RS35640 0.47 -0.8 3,589,021 - BPHYT_RS35640 0.56 +1.2 3,589,081 - BPHYT_RS35640 0.69 +1.5 3,589,103 - BPHYT_RS35640 0.74 -1.1 3,589,103 - BPHYT_RS35640 0.74 +0.6 3,589,206 - -1.5 3,589,435 + +2.9
Or see this region's nucleotide sequence