Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35335 and BPHYT_RS35340 are separated by 40 nucleotides BPHYT_RS35340 and BPHYT_RS35345 overlap by 4 nucleotides BPHYT_RS35345 and BPHYT_RS35350 are separated by 75 nucleotides
BPHYT_RS35335: BPHYT_RS35335 - cobalamin biosynthesis protein, at 3,526,712 to 3,527,794
_RS35335
BPHYT_RS35340: BPHYT_RS35340 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 3,527,835 to 3,528,425
_RS35340
BPHYT_RS35345: BPHYT_RS35345 - decarboxylase UbiD, at 3,528,422 to 3,529,816
_RS35345
BPHYT_RS35350: BPHYT_RS35350 - amidohydrolase, at 3,529,892 to 3,530,818
_RS35350
Position (kb)
3528
3529
3530 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3527.697 kb on + strand at 3527.833 kb on + strand at 3527.845 kb on + strand at 3527.846 kb on - strand at 3528.015 kb on + strand, within BPHYT_RS35340 at 3528.066 kb on + strand, within BPHYT_RS35340 at 3528.236 kb on - strand, within BPHYT_RS35340 at 3528.361 kb on - strand, within BPHYT_RS35340 at 3528.604 kb on + strand, within BPHYT_RS35345 at 3529.399 kb on - strand, within BPHYT_RS35345 at 3529.536 kb on + strand, within BPHYT_RS35345 at 3529.589 kb on - strand, within BPHYT_RS35345 at 3529.727 kb on - strand at 3529.817 kb on - strand at 3530.536 kb on - strand, within BPHYT_RS35350 at 3530.599 kb on - strand, within BPHYT_RS35350 at 3530.709 kb on + strand, within BPHYT_RS35350 at 3530.709 kb on + strand, within BPHYT_RS35350 at 3530.709 kb on + strand, within BPHYT_RS35350
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,527,697 + +0.1 3,527,833 + +0.3 3,527,845 + +1.0 3,527,846 - +1.7 3,528,015 + BPHYT_RS35340 0.30 -0.4 3,528,066 + BPHYT_RS35340 0.39 -0.9 3,528,236 - BPHYT_RS35340 0.68 -0.8 3,528,361 - BPHYT_RS35340 0.89 -1.0 3,528,604 + BPHYT_RS35345 0.13 +0.4 3,529,399 - BPHYT_RS35345 0.70 -0.8 3,529,536 + BPHYT_RS35345 0.80 -0.2 3,529,589 - BPHYT_RS35345 0.84 -1.1 3,529,727 - +1.7 3,529,817 - +0.8 3,530,536 - BPHYT_RS35350 0.69 +0.1 3,530,599 - BPHYT_RS35350 0.76 +0.3 3,530,709 + BPHYT_RS35350 0.88 -1.8 3,530,709 + BPHYT_RS35350 0.88 +0.4 3,530,709 + BPHYT_RS35350 0.88 +3.6
Or see this region's nucleotide sequence