Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35325

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35315 and BPHYT_RS35320 are separated by 116 nucleotidesBPHYT_RS35320 and BPHYT_RS35325 are separated by 20 nucleotidesBPHYT_RS35325 and BPHYT_RS35330 are separated by 74 nucleotides BPHYT_RS35315: BPHYT_RS35315 - threonyl-tRNA synthetase, at 3,521,733 to 3,522,935 _RS35315 BPHYT_RS35320: BPHYT_RS35320 - Asp/Glu/hydantoin racemase, at 3,523,052 to 3,523,801 _RS35320 BPHYT_RS35325: BPHYT_RS35325 - amidohydrolase, at 3,523,822 to 3,525,207 _RS35325 BPHYT_RS35330: BPHYT_RS35330 - purine permease, at 3,525,282 to 3,526,622 _RS35330 Position (kb) 3523 3524 3525 3526Strain fitness (log2 ratio) -1 0 1 2at 3522.833 kb on + strandat 3523.180 kb on + strand, within BPHYT_RS35320at 3523.324 kb on + strand, within BPHYT_RS35320at 3523.382 kb on - strand, within BPHYT_RS35320at 3523.862 kb on + strandat 3523.934 kb on + strandat 3524.485 kb on - strand, within BPHYT_RS35325at 3524.485 kb on - strand, within BPHYT_RS35325at 3524.485 kb on - strand, within BPHYT_RS35325at 3524.921 kb on - strand, within BPHYT_RS35325

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,522,833 + -0.1
3,523,180 + BPHYT_RS35320 0.17 +2.2
3,523,324 + BPHYT_RS35320 0.36 +0.9
3,523,382 - BPHYT_RS35320 0.44 +0.7
3,523,862 + +1.5
3,523,934 + -1.2
3,524,485 - BPHYT_RS35325 0.48 -1.7
3,524,485 - BPHYT_RS35325 0.48 -1.1
3,524,485 - BPHYT_RS35325 0.48 +0.7
3,524,921 - BPHYT_RS35325 0.79 +0.8

Or see this region's nucleotide sequence