Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35060 and BPHYT_RS35065 are separated by 165 nucleotides BPHYT_RS35065 and BPHYT_RS35075 are separated by 654 nucleotides BPHYT_RS35075 and BPHYT_RS35080 are separated by 31 nucleotides
BPHYT_RS35060: BPHYT_RS35060 - glycosyl transferase, at 3,468,189 to 3,469,640
_RS35060
BPHYT_RS35065: BPHYT_RS35065 - Rieske (2Fe-2S) protein, at 3,469,806 to 3,471,026
_RS35065
BPHYT_RS35075: BPHYT_RS35075 - rubredoxin, at 3,471,681 to 3,471,827
_RS35075
BPHYT_RS35080: BPHYT_RS35080 - short-chain dehydrogenase, at 3,471,859 to 3,472,713
_RS35080
Position (kb)
3469
3470
3471
3472 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3468.936 kb on - strand, within BPHYT_RS35060 at 3469.059 kb on + strand, within BPHYT_RS35060 at 3469.315 kb on - strand, within BPHYT_RS35060 at 3469.429 kb on - strand, within BPHYT_RS35060 at 3469.716 kb on + strand at 3469.731 kb on + strand at 3469.735 kb on - strand at 3469.735 kb on - strand at 3469.805 kb on + strand at 3469.805 kb on + strand at 3469.805 kb on + strand at 3469.806 kb on - strand at 3469.806 kb on - strand at 3469.806 kb on - strand at 3469.806 kb on - strand at 3469.806 kb on - strand at 3469.826 kb on - strand at 3469.847 kb on - strand at 3469.847 kb on - strand at 3469.962 kb on + strand, within BPHYT_RS35065 at 3469.968 kb on + strand, within BPHYT_RS35065 at 3469.969 kb on - strand, within BPHYT_RS35065 at 3470.166 kb on + strand, within BPHYT_RS35065 at 3470.166 kb on + strand, within BPHYT_RS35065 at 3470.167 kb on - strand, within BPHYT_RS35065 at 3470.167 kb on - strand, within BPHYT_RS35065 at 3470.199 kb on + strand, within BPHYT_RS35065 at 3470.239 kb on - strand, within BPHYT_RS35065 at 3470.343 kb on + strand, within BPHYT_RS35065 at 3470.382 kb on + strand, within BPHYT_RS35065 at 3470.592 kb on - strand, within BPHYT_RS35065 at 3470.619 kb on + strand, within BPHYT_RS35065 at 3470.625 kb on + strand, within BPHYT_RS35065 at 3470.685 kb on + strand, within BPHYT_RS35065 at 3470.686 kb on - strand, within BPHYT_RS35065 at 3470.686 kb on - strand, within BPHYT_RS35065 at 3470.947 kb on + strand at 3471.048 kb on + strand at 3471.048 kb on + strand at 3471.048 kb on + strand at 3471.049 kb on - strand at 3471.049 kb on - strand at 3471.105 kb on + strand at 3471.105 kb on + strand at 3471.106 kb on - strand at 3471.106 kb on - strand at 3471.125 kb on + strand at 3471.185 kb on + strand at 3471.418 kb on - strand at 3471.436 kb on + strand at 3471.437 kb on - strand at 3471.437 kb on - strand at 3471.612 kb on + strand at 3471.826 kb on - strand at 3471.847 kb on + strand at 3471.848 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,468,936 - BPHYT_RS35060 0.51 -1.1 3,469,059 + BPHYT_RS35060 0.60 -0.5 3,469,315 - BPHYT_RS35060 0.78 -0.5 3,469,429 - BPHYT_RS35060 0.85 -0.7 3,469,716 + +3.2 3,469,731 + -0.4 3,469,735 - -2.1 3,469,735 - -1.9 3,469,805 + +0.1 3,469,805 + -1.2 3,469,805 + -0.0 3,469,806 - -0.4 3,469,806 - -0.6 3,469,806 - -0.7 3,469,806 - +0.3 3,469,806 - +0.4 3,469,826 - +0.2 3,469,847 - -0.2 3,469,847 - +0.9 3,469,962 + BPHYT_RS35065 0.13 +0.7 3,469,968 + BPHYT_RS35065 0.13 +0.5 3,469,969 - BPHYT_RS35065 0.13 -1.0 3,470,166 + BPHYT_RS35065 0.29 +1.6 3,470,166 + BPHYT_RS35065 0.29 +0.3 3,470,167 - BPHYT_RS35065 0.30 +0.2 3,470,167 - BPHYT_RS35065 0.30 -0.2 3,470,199 + BPHYT_RS35065 0.32 -0.2 3,470,239 - BPHYT_RS35065 0.35 +0.7 3,470,343 + BPHYT_RS35065 0.44 -1.2 3,470,382 + BPHYT_RS35065 0.47 -1.0 3,470,592 - BPHYT_RS35065 0.64 +0.3 3,470,619 + BPHYT_RS35065 0.67 -1.0 3,470,625 + BPHYT_RS35065 0.67 -0.7 3,470,685 + BPHYT_RS35065 0.72 -3.1 3,470,686 - BPHYT_RS35065 0.72 +0.3 3,470,686 - BPHYT_RS35065 0.72 +0.1 3,470,947 + +2.3 3,471,048 + +0.2 3,471,048 + +1.2 3,471,048 + -0.4 3,471,049 - -0.1 3,471,049 - +0.5 3,471,105 + -0.3 3,471,105 + -1.2 3,471,106 - +2.1 3,471,106 - +0.2 3,471,125 + -1.7 3,471,185 + -1.2 3,471,418 - -0.4 3,471,436 + +0.3 3,471,437 - -1.9 3,471,437 - -0.5 3,471,612 + +0.2 3,471,826 - -0.2 3,471,847 + +1.2 3,471,848 - +0.5
Or see this region's nucleotide sequence