Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34980 and BPHYT_RS34985 are separated by 26 nucleotides BPHYT_RS34985 and BPHYT_RS34990 overlap by 8 nucleotides BPHYT_RS34990 and BPHYT_RS34995 are separated by 25 nucleotides
BPHYT_RS34980: BPHYT_RS34980 - aldehyde dehydrogenase, at 3,446,907 to 3,448,397
_RS34980
BPHYT_RS34985: BPHYT_RS34985 - glyoxalase, at 3,448,424 to 3,449,389
_RS34985
BPHYT_RS34990: BPHYT_RS34990 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 3,449,382 to 3,450,647
_RS34990
BPHYT_RS34995: BPHYT_RS34995 - MFS transporter, at 3,450,673 to 3,452,076
_RS34995
Position (kb)
3449
3450
3451 Strain fitness (log2 ratio)
-2
-1
0
1 at 3448.395 kb on + strand at 3448.854 kb on - strand, within BPHYT_RS34985 at 3449.113 kb on + strand, within BPHYT_RS34985 at 3449.113 kb on + strand, within BPHYT_RS34985 at 3449.553 kb on + strand, within BPHYT_RS34990 at 3449.554 kb on - strand, within BPHYT_RS34990 at 3450.653 kb on + strand at 3450.656 kb on - strand at 3450.657 kb on - strand at 3451.050 kb on + strand, within BPHYT_RS34995 at 3451.579 kb on + strand, within BPHYT_RS34995
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,448,395 + +0.4 3,448,854 - BPHYT_RS34985 0.45 +0.5 3,449,113 + BPHYT_RS34985 0.71 -0.4 3,449,113 + BPHYT_RS34985 0.71 -0.1 3,449,553 + BPHYT_RS34990 0.14 +1.4 3,449,554 - BPHYT_RS34990 0.14 +1.1 3,450,653 + -0.9 3,450,656 - +0.8 3,450,657 - -0.8 3,451,050 + BPHYT_RS34995 0.27 -2.5 3,451,579 + BPHYT_RS34995 0.65 -0.0
Or see this region's nucleotide sequence