Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34775

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34765 and BPHYT_RS34770 overlap by 8 nucleotidesBPHYT_RS34770 and BPHYT_RS34775 overlap by 4 nucleotidesBPHYT_RS34775 and BPHYT_RS34780 are separated by 277 nucleotides BPHYT_RS34765: BPHYT_RS34765 - chemotaxis protein, at 3,404,768 to 3,405,136 _RS34765 BPHYT_RS34770: BPHYT_RS34770 - chemotaxis protein, at 3,405,129 to 3,405,752 _RS34770 BPHYT_RS34775: BPHYT_RS34775 - diguanylate cyclase, at 3,405,749 to 3,406,687 _RS34775 BPHYT_RS34780: BPHYT_RS34780 - phytoene synthase, at 3,406,965 to 3,407,813 _RS34780 Position (kb) 3405 3406 3407Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3404.779 kb on + strandat 3404.779 kb on + strandat 3404.779 kb on + strandat 3404.780 kb on - strandat 3404.861 kb on + strand, within BPHYT_RS34765at 3404.862 kb on - strand, within BPHYT_RS34765at 3404.954 kb on + strand, within BPHYT_RS34765at 3405.100 kb on - strandat 3405.103 kb on - strandat 3405.103 kb on - strandat 3405.128 kb on + strandat 3405.128 kb on + strandat 3405.128 kb on + strandat 3405.128 kb on + strandat 3405.129 kb on - strandat 3405.129 kb on - strandat 3405.429 kb on + strand, within BPHYT_RS34770at 3405.430 kb on - strand, within BPHYT_RS34770at 3405.444 kb on + strand, within BPHYT_RS34770at 3405.634 kb on + strand, within BPHYT_RS34770at 3405.634 kb on + strand, within BPHYT_RS34770at 3405.634 kb on + strand, within BPHYT_RS34770at 3405.635 kb on - strand, within BPHYT_RS34770at 3405.714 kb on - strandat 3405.748 kb on + strandat 3405.748 kb on + strandat 3405.749 kb on - strandat 3405.906 kb on - strand, within BPHYT_RS34775at 3405.906 kb on - strand, within BPHYT_RS34775at 3406.194 kb on - strand, within BPHYT_RS34775at 3406.262 kb on + strand, within BPHYT_RS34775at 3406.641 kb on - strandat 3406.641 kb on - strandat 3406.643 kb on - strandat 3406.834 kb on + strandat 3406.836 kb on + strandat 3406.837 kb on - strandat 3406.837 kb on - strandat 3407.091 kb on + strand, within BPHYT_RS34780at 3407.193 kb on + strand, within BPHYT_RS34780at 3407.346 kb on + strand, within BPHYT_RS34780at 3407.347 kb on - strand, within BPHYT_RS34780at 3407.393 kb on - strand, within BPHYT_RS34780

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,404,779 + -0.5
3,404,779 + +2.1
3,404,779 + +0.7
3,404,780 - -1.2
3,404,861 + BPHYT_RS34765 0.25 -0.3
3,404,862 - BPHYT_RS34765 0.25 +1.2
3,404,954 + BPHYT_RS34765 0.50 -0.5
3,405,100 - +0.0
3,405,103 - +0.3
3,405,103 - -0.5
3,405,128 + -0.1
3,405,128 + +1.2
3,405,128 + -0.9
3,405,128 + -2.5
3,405,129 - -2.8
3,405,129 - -1.3
3,405,429 + BPHYT_RS34770 0.48 -0.0
3,405,430 - BPHYT_RS34770 0.48 -0.2
3,405,444 + BPHYT_RS34770 0.50 -0.2
3,405,634 + BPHYT_RS34770 0.81 +0.3
3,405,634 + BPHYT_RS34770 0.81 -1.5
3,405,634 + BPHYT_RS34770 0.81 -0.5
3,405,635 - BPHYT_RS34770 0.81 +0.9
3,405,714 - -0.1
3,405,748 + -0.6
3,405,748 + +1.2
3,405,749 - +1.3
3,405,906 - BPHYT_RS34775 0.17 -1.4
3,405,906 - BPHYT_RS34775 0.17 +1.1
3,406,194 - BPHYT_RS34775 0.47 +0.8
3,406,262 + BPHYT_RS34775 0.55 +0.8
3,406,641 - -0.2
3,406,641 - -1.0
3,406,643 - -2.9
3,406,834 + -1.3
3,406,836 + +0.1
3,406,837 - -3.3
3,406,837 - -2.6
3,407,091 + BPHYT_RS34780 0.15 +1.2
3,407,193 + BPHYT_RS34780 0.27 -2.7
3,407,346 + BPHYT_RS34780 0.45 -3.2
3,407,347 - BPHYT_RS34780 0.45 -1.0
3,407,393 - BPHYT_RS34780 0.50 +0.6

Or see this region's nucleotide sequence