Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34765 and BPHYT_RS34770 overlap by 8 nucleotides BPHYT_RS34770 and BPHYT_RS34775 overlap by 4 nucleotides BPHYT_RS34775 and BPHYT_RS34780 are separated by 277 nucleotides
BPHYT_RS34765: BPHYT_RS34765 - chemotaxis protein, at 3,404,768 to 3,405,136
_RS34765
BPHYT_RS34770: BPHYT_RS34770 - chemotaxis protein, at 3,405,129 to 3,405,752
_RS34770
BPHYT_RS34775: BPHYT_RS34775 - diguanylate cyclase, at 3,405,749 to 3,406,687
_RS34775
BPHYT_RS34780: BPHYT_RS34780 - phytoene synthase, at 3,406,965 to 3,407,813
_RS34780
Position (kb)
3405
3406
3407 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3404.779 kb on + strand at 3404.779 kb on + strand at 3404.779 kb on + strand at 3404.780 kb on - strand at 3404.861 kb on + strand, within BPHYT_RS34765 at 3404.862 kb on - strand, within BPHYT_RS34765 at 3404.954 kb on + strand, within BPHYT_RS34765 at 3405.100 kb on - strand at 3405.103 kb on - strand at 3405.103 kb on - strand at 3405.128 kb on + strand at 3405.128 kb on + strand at 3405.128 kb on + strand at 3405.128 kb on + strand at 3405.129 kb on - strand at 3405.129 kb on - strand at 3405.429 kb on + strand, within BPHYT_RS34770 at 3405.430 kb on - strand, within BPHYT_RS34770 at 3405.444 kb on + strand, within BPHYT_RS34770 at 3405.634 kb on + strand, within BPHYT_RS34770 at 3405.634 kb on + strand, within BPHYT_RS34770 at 3405.634 kb on + strand, within BPHYT_RS34770 at 3405.635 kb on - strand, within BPHYT_RS34770 at 3405.714 kb on - strand at 3405.748 kb on + strand at 3405.748 kb on + strand at 3405.749 kb on - strand at 3405.906 kb on - strand, within BPHYT_RS34775 at 3405.906 kb on - strand, within BPHYT_RS34775 at 3406.194 kb on - strand, within BPHYT_RS34775 at 3406.262 kb on + strand, within BPHYT_RS34775 at 3406.641 kb on - strand at 3406.641 kb on - strand at 3406.643 kb on - strand at 3406.834 kb on + strand at 3406.836 kb on + strand at 3406.837 kb on - strand at 3406.837 kb on - strand at 3407.091 kb on + strand, within BPHYT_RS34780 at 3407.193 kb on + strand, within BPHYT_RS34780 at 3407.346 kb on + strand, within BPHYT_RS34780 at 3407.347 kb on - strand, within BPHYT_RS34780 at 3407.393 kb on - strand, within BPHYT_RS34780
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,404,779 + -0.5 3,404,779 + +2.1 3,404,779 + +0.7 3,404,780 - -1.2 3,404,861 + BPHYT_RS34765 0.25 -0.3 3,404,862 - BPHYT_RS34765 0.25 +1.2 3,404,954 + BPHYT_RS34765 0.50 -0.5 3,405,100 - +0.0 3,405,103 - +0.3 3,405,103 - -0.5 3,405,128 + -0.1 3,405,128 + +1.2 3,405,128 + -0.9 3,405,128 + -2.5 3,405,129 - -2.8 3,405,129 - -1.3 3,405,429 + BPHYT_RS34770 0.48 -0.0 3,405,430 - BPHYT_RS34770 0.48 -0.2 3,405,444 + BPHYT_RS34770 0.50 -0.2 3,405,634 + BPHYT_RS34770 0.81 +0.3 3,405,634 + BPHYT_RS34770 0.81 -1.5 3,405,634 + BPHYT_RS34770 0.81 -0.5 3,405,635 - BPHYT_RS34770 0.81 +0.9 3,405,714 - -0.1 3,405,748 + -0.6 3,405,748 + +1.2 3,405,749 - +1.3 3,405,906 - BPHYT_RS34775 0.17 -1.4 3,405,906 - BPHYT_RS34775 0.17 +1.1 3,406,194 - BPHYT_RS34775 0.47 +0.8 3,406,262 + BPHYT_RS34775 0.55 +0.8 3,406,641 - -0.2 3,406,641 - -1.0 3,406,643 - -2.9 3,406,834 + -1.3 3,406,836 + +0.1 3,406,837 - -3.3 3,406,837 - -2.6 3,407,091 + BPHYT_RS34780 0.15 +1.2 3,407,193 + BPHYT_RS34780 0.27 -2.7 3,407,346 + BPHYT_RS34780 0.45 -3.2 3,407,347 - BPHYT_RS34780 0.45 -1.0 3,407,393 - BPHYT_RS34780 0.50 +0.6
Or see this region's nucleotide sequence