Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34635 and BPHYT_RS34640 are separated by 13 nucleotides BPHYT_RS34640 and BPHYT_RS34645 are separated by 108 nucleotides BPHYT_RS34645 and BPHYT_RS34650 are separated by 97 nucleotides
BPHYT_RS34635: BPHYT_RS34635 - 50S ribosomal protein L21, at 3,378,788 to 3,379,267
_RS34635
BPHYT_RS34640: BPHYT_RS34640 - TetR family transcriptional regulator, at 3,379,281 to 3,379,946
_RS34640
BPHYT_RS34645: BPHYT_RS34645 - hypothetical protein, at 3,380,055 to 3,380,705
_RS34645
BPHYT_RS34650: BPHYT_RS34650 - l-threonine 3-dehydrogenase, at 3,380,803 to 3,381,834
_RS34650
Position (kb)
3380
3381 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3379.270 kb on + strand at 3379.271 kb on - strand at 3379.793 kb on - strand, within BPHYT_RS34640 at 3379.865 kb on - strand, within BPHYT_RS34640 at 3379.948 kb on + strand at 3379.949 kb on - strand at 3380.019 kb on + strand at 3380.020 kb on - strand at 3380.020 kb on - strand at 3380.148 kb on - strand, within BPHYT_RS34645 at 3380.148 kb on - strand, within BPHYT_RS34645 at 3380.154 kb on - strand, within BPHYT_RS34645 at 3380.247 kb on + strand, within BPHYT_RS34645 at 3380.248 kb on - strand, within BPHYT_RS34645 at 3380.635 kb on - strand, within BPHYT_RS34645 at 3380.654 kb on + strand at 3380.800 kb on - strand at 3381.094 kb on - strand, within BPHYT_RS34650 at 3381.094 kb on - strand, within BPHYT_RS34650 at 3381.240 kb on - strand, within BPHYT_RS34650 at 3381.642 kb on - strand, within BPHYT_RS34650
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,379,270 + -3.1 3,379,271 - +0.2 3,379,793 - BPHYT_RS34640 0.77 -0.6 3,379,865 - BPHYT_RS34640 0.88 -0.6 3,379,948 + +0.4 3,379,949 - -0.7 3,380,019 + -0.8 3,380,020 - +0.5 3,380,020 - +0.1 3,380,148 - BPHYT_RS34645 0.14 -0.3 3,380,148 - BPHYT_RS34645 0.14 +0.5 3,380,154 - BPHYT_RS34645 0.15 +2.0 3,380,247 + BPHYT_RS34645 0.29 +2.4 3,380,248 - BPHYT_RS34645 0.30 -1.0 3,380,635 - BPHYT_RS34645 0.89 +2.1 3,380,654 + -0.1 3,380,800 - -1.1 3,381,094 - BPHYT_RS34650 0.28 +0.4 3,381,094 - BPHYT_RS34650 0.28 -1.0 3,381,240 - BPHYT_RS34650 0.42 -0.3 3,381,642 - BPHYT_RS34650 0.81 +1.4
Or see this region's nucleotide sequence