Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34510

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34505 and BPHYT_RS34510 are separated by 307 nucleotidesBPHYT_RS34510 and BPHYT_RS34515 are separated by 12 nucleotides BPHYT_RS34505: BPHYT_RS34505 - hypothetical protein, at 3,348,489 to 3,349,061 _RS34505 BPHYT_RS34510: BPHYT_RS34510 - sodium:proton antiporter, at 3,349,369 to 3,350,955 _RS34510 BPHYT_RS34515: BPHYT_RS34515 - MFS transporter, at 3,350,968 to 3,352,299 _RS34515 Position (kb) 3349 3350 3351Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 3348.672 kb on - strand, within BPHYT_RS34505at 3348.747 kb on + strand, within BPHYT_RS34505at 3348.748 kb on - strand, within BPHYT_RS34505at 3349.008 kb on + strandat 3349.110 kb on + strandat 3349.111 kb on - strandat 3349.111 kb on - strandat 3349.123 kb on + strandat 3349.127 kb on - strandat 3349.356 kb on - strandat 3349.582 kb on + strand, within BPHYT_RS34510at 3349.993 kb on + strand, within BPHYT_RS34510at 3350.050 kb on + strand, within BPHYT_RS34510at 3350.050 kb on + strand, within BPHYT_RS34510at 3350.051 kb on - strand, within BPHYT_RS34510at 3350.252 kb on + strand, within BPHYT_RS34510at 3350.252 kb on + strand, within BPHYT_RS34510at 3350.370 kb on - strand, within BPHYT_RS34510at 3350.455 kb on - strand, within BPHYT_RS34510at 3350.564 kb on + strand, within BPHYT_RS34510at 3350.564 kb on + strand, within BPHYT_RS34510at 3350.565 kb on - strand, within BPHYT_RS34510at 3350.735 kb on - strand, within BPHYT_RS34510at 3350.735 kb on - strand, within BPHYT_RS34510at 3350.740 kb on + strand, within BPHYT_RS34510at 3350.741 kb on - strand, within BPHYT_RS34510at 3350.810 kb on - strandat 3351.011 kb on + strandat 3351.011 kb on + strandat 3351.056 kb on + strandat 3351.056 kb on + strandat 3351.056 kb on + strandat 3351.056 kb on + strandat 3351.056 kb on + strandat 3351.057 kb on - strandat 3351.057 kb on - strandat 3351.380 kb on - strand, within BPHYT_RS34515at 3351.402 kb on + strand, within BPHYT_RS34515at 3351.403 kb on - strand, within BPHYT_RS34515at 3351.404 kb on + strand, within BPHYT_RS34515at 3351.404 kb on + strand, within BPHYT_RS34515at 3351.405 kb on - strand, within BPHYT_RS34515at 3351.515 kb on + strand, within BPHYT_RS34515at 3351.516 kb on - strand, within BPHYT_RS34515at 3351.516 kb on - strand, within BPHYT_RS34515at 3351.841 kb on - strand, within BPHYT_RS34515at 3351.841 kb on - strand, within BPHYT_RS34515at 3351.841 kb on - strand, within BPHYT_RS34515at 3351.878 kb on + strand, within BPHYT_RS34515

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,348,672 - BPHYT_RS34505 0.32 +0.2
3,348,747 + BPHYT_RS34505 0.45 -0.3
3,348,748 - BPHYT_RS34505 0.45 +1.0
3,349,008 + -0.5
3,349,110 + +0.3
3,349,111 - -2.0
3,349,111 - -0.5
3,349,123 + +0.1
3,349,127 - -0.2
3,349,356 - -0.4
3,349,582 + BPHYT_RS34510 0.13 +0.0
3,349,993 + BPHYT_RS34510 0.39 -0.1
3,350,050 + BPHYT_RS34510 0.43 -0.0
3,350,050 + BPHYT_RS34510 0.43 +0.3
3,350,051 - BPHYT_RS34510 0.43 -0.7
3,350,252 + BPHYT_RS34510 0.56 -0.7
3,350,252 + BPHYT_RS34510 0.56 -0.9
3,350,370 - BPHYT_RS34510 0.63 +6.1
3,350,455 - BPHYT_RS34510 0.68 +0.9
3,350,564 + BPHYT_RS34510 0.75 +0.1
3,350,564 + BPHYT_RS34510 0.75 -0.4
3,350,565 - BPHYT_RS34510 0.75 -0.4
3,350,735 - BPHYT_RS34510 0.86 +0.1
3,350,735 - BPHYT_RS34510 0.86 -0.6
3,350,740 + BPHYT_RS34510 0.86 +0.2
3,350,741 - BPHYT_RS34510 0.86 +0.3
3,350,810 - +0.9
3,351,011 + +1.1
3,351,011 + +0.1
3,351,056 + +0.1
3,351,056 + +0.1
3,351,056 + -1.6
3,351,056 + +1.1
3,351,056 + +0.2
3,351,057 - -0.1
3,351,057 - -0.3
3,351,380 - BPHYT_RS34515 0.31 -0.3
3,351,402 + BPHYT_RS34515 0.33 +1.4
3,351,403 - BPHYT_RS34515 0.33 +0.2
3,351,404 + BPHYT_RS34515 0.33 -1.4
3,351,404 + BPHYT_RS34515 0.33 +1.1
3,351,405 - BPHYT_RS34515 0.33 -2.8
3,351,515 + BPHYT_RS34515 0.41 +1.0
3,351,516 - BPHYT_RS34515 0.41 -0.4
3,351,516 - BPHYT_RS34515 0.41 +0.0
3,351,841 - BPHYT_RS34515 0.66 -1.4
3,351,841 - BPHYT_RS34515 0.66 -1.7
3,351,841 - BPHYT_RS34515 0.66 -0.8
3,351,878 + BPHYT_RS34515 0.68 +0.5

Or see this region's nucleotide sequence