Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34445 and BPHYT_RS34450 are separated by 253 nucleotides BPHYT_RS34450 and BPHYT_RS34455 are separated by 143 nucleotides BPHYT_RS34455 and BPHYT_RS34460 overlap by 4 nucleotides
BPHYT_RS34445: BPHYT_RS34445 - GntR family transcriptional regulator, at 3,336,732 to 3,337,457
_RS34445
BPHYT_RS34450: BPHYT_RS34450 - LysR family transcriptional regulator, at 3,337,711 to 3,338,661
_RS34450
BPHYT_RS34455: BPHYT_RS34455 - arginine ABC transporter ATP-binding protein, at 3,338,805 to 3,339,530
_RS34455
BPHYT_RS34460: BPHYT_RS34460 - glutamate/aspartate transporter permease GltK, at 3,339,527 to 3,340,204
_RS34460
Position (kb)
3337
3338
3339 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3337.162 kb on - strand, within BPHYT_RS34445 at 3337.162 kb on - strand, within BPHYT_RS34445 at 3337.162 kb on - strand, within BPHYT_RS34445 at 3337.162 kb on - strand, within BPHYT_RS34445 at 3337.230 kb on + strand, within BPHYT_RS34445 at 3337.231 kb on - strand, within BPHYT_RS34445 at 3337.392 kb on - strand at 3337.547 kb on + strand at 3337.579 kb on + strand at 3337.579 kb on + strand at 3337.713 kb on - strand at 3337.713 kb on - strand at 3337.721 kb on - strand at 3337.919 kb on - strand, within BPHYT_RS34450 at 3337.954 kb on - strand, within BPHYT_RS34450 at 3338.231 kb on + strand, within BPHYT_RS34450 at 3338.231 kb on + strand, within BPHYT_RS34450 at 3338.534 kb on - strand, within BPHYT_RS34450 at 3338.554 kb on - strand, within BPHYT_RS34450 at 3339.500 kb on - strand at 3339.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,337,162 - BPHYT_RS34445 0.59 -1.2 3,337,162 - BPHYT_RS34445 0.59 -0.2 3,337,162 - BPHYT_RS34445 0.59 -0.4 3,337,162 - BPHYT_RS34445 0.59 -2.5 3,337,230 + BPHYT_RS34445 0.69 +0.0 3,337,231 - BPHYT_RS34445 0.69 +1.0 3,337,392 - -0.7 3,337,547 + -0.7 3,337,579 + -1.2 3,337,579 + +0.5 3,337,713 - +0.5 3,337,713 - +0.2 3,337,721 - -0.4 3,337,919 - BPHYT_RS34450 0.22 -0.8 3,337,954 - BPHYT_RS34450 0.26 +2.2 3,338,231 + BPHYT_RS34450 0.55 +0.2 3,338,231 + BPHYT_RS34450 0.55 +1.2 3,338,534 - BPHYT_RS34450 0.87 +0.3 3,338,554 - BPHYT_RS34450 0.89 +0.4 3,339,500 - -0.8 3,339,507 - +0.7
Or see this region's nucleotide sequence