Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34285 and BPHYT_RS34290 are separated by 115 nucleotides BPHYT_RS34290 and BPHYT_RS34295 are separated by 77 nucleotides BPHYT_RS34295 and BPHYT_RS34300 are separated by 342 nucleotides
BPHYT_RS34285: BPHYT_RS34285 - fimbrial biogenesis outer membrane usher protein, at 3,295,676 to 3,298,336
_RS34285
BPHYT_RS34290: BPHYT_RS34290 - molecular chaperone EcpD, at 3,298,452 to 3,299,195
_RS34290
BPHYT_RS34295: BPHYT_RS34295 - fimbrial protein, at 3,299,273 to 3,299,830
_RS34295
BPHYT_RS34300: BPHYT_RS34300 - major facilitator transporter, at 3,300,173 to 3,301,555
_RS34300
Position (kb)
3299
3300 Strain fitness (log2 ratio)
-1
0
1 at 3298.410 kb on + strand at 3298.605 kb on - strand, within BPHYT_RS34290 at 3298.651 kb on + strand, within BPHYT_RS34290 at 3298.795 kb on + strand, within BPHYT_RS34290 at 3298.907 kb on - strand, within BPHYT_RS34290 at 3298.961 kb on + strand, within BPHYT_RS34290 at 3299.041 kb on - strand, within BPHYT_RS34290 at 3299.352 kb on + strand, within BPHYT_RS34295 at 3299.885 kb on + strand at 3300.058 kb on + strand at 3300.151 kb on - strand at 3300.682 kb on + strand, within BPHYT_RS34300 at 3300.683 kb on - strand, within BPHYT_RS34300
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,298,410 + +0.1 3,298,605 - BPHYT_RS34290 0.21 -0.6 3,298,651 + BPHYT_RS34290 0.27 +0.0 3,298,795 + BPHYT_RS34290 0.46 -0.5 3,298,907 - BPHYT_RS34290 0.61 -0.7 3,298,961 + BPHYT_RS34290 0.68 +0.4 3,299,041 - BPHYT_RS34290 0.79 +0.1 3,299,352 + BPHYT_RS34295 0.14 -0.8 3,299,885 + -0.5 3,300,058 + +0.2 3,300,151 - -0.3 3,300,682 + BPHYT_RS34300 0.37 -0.7 3,300,683 - BPHYT_RS34300 0.37 -1.1
Or see this region's nucleotide sequence