Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34250

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34245 and BPHYT_RS34250 overlap by 4 nucleotidesBPHYT_RS34250 and BPHYT_RS34255 are separated by 273 nucleotides BPHYT_RS34245: BPHYT_RS34245 - ABC transporter for L-rhamnose/L-fucose/xylitol, ATPase component (from data), at 3,284,317 to 3,285,849 _RS34245 BPHYT_RS34250: BPHYT_RS34250 - ABC transporter for L-rhamnose/L-fucose/xylitol, substrate-binding component (from data), at 3,285,846 to 3,286,895 _RS34250 BPHYT_RS34255: BPHYT_RS34255 - IclR family transcriptional regulator, at 3,287,169 to 3,287,957 _RS34255 Position (kb) 3285 3286 3287Strain fitness (log2 ratio) -1 0 1 2 3at 3284.934 kb on + strand, within BPHYT_RS34245at 3285.567 kb on - strand, within BPHYT_RS34245at 3285.860 kb on + strandat 3286.216 kb on + strand, within BPHYT_RS34250at 3286.216 kb on + strand, within BPHYT_RS34250at 3286.429 kb on + strand, within BPHYT_RS34250at 3286.430 kb on - strand, within BPHYT_RS34250at 3286.711 kb on + strand, within BPHYT_RS34250at 3286.711 kb on + strand, within BPHYT_RS34250at 3286.712 kb on - strand, within BPHYT_RS34250at 3286.720 kb on + strand, within BPHYT_RS34250at 3286.721 kb on - strand, within BPHYT_RS34250at 3286.910 kb on - strandat 3286.971 kb on - strandat 3286.971 kb on - strandat 3287.005 kb on + strandat 3287.018 kb on - strandat 3287.018 kb on - strandat 3287.020 kb on + strandat 3287.026 kb on + strandat 3287.026 kb on + strandat 3287.026 kb on + strandat 3287.027 kb on - strandat 3287.027 kb on - strandat 3287.027 kb on - strandat 3287.028 kb on + strandat 3287.029 kb on - strandat 3287.120 kb on + strandat 3287.137 kb on + strandat 3287.320 kb on - strand, within BPHYT_RS34255at 3287.320 kb on - strand, within BPHYT_RS34255at 3287.352 kb on + strand, within BPHYT_RS34255at 3287.403 kb on + strand, within BPHYT_RS34255at 3287.404 kb on - strand, within BPHYT_RS34255at 3287.510 kb on - strand, within BPHYT_RS34255at 3287.512 kb on - strand, within BPHYT_RS34255at 3287.512 kb on - strand, within BPHYT_RS34255at 3287.855 kb on - strand, within BPHYT_RS34255at 3287.871 kb on + strand, within BPHYT_RS34255at 3287.872 kb on - strand, within BPHYT_RS34255

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,284,934 + BPHYT_RS34245 0.40 +0.7
3,285,567 - BPHYT_RS34245 0.82 +0.6
3,285,860 + +0.2
3,286,216 + BPHYT_RS34250 0.35 +1.9
3,286,216 + BPHYT_RS34250 0.35 +2.2
3,286,429 + BPHYT_RS34250 0.56 +1.7
3,286,430 - BPHYT_RS34250 0.56 +1.0
3,286,711 + BPHYT_RS34250 0.82 +1.7
3,286,711 + BPHYT_RS34250 0.82 +0.2
3,286,712 - BPHYT_RS34250 0.82 +1.2
3,286,720 + BPHYT_RS34250 0.83 +2.4
3,286,721 - BPHYT_RS34250 0.83 +1.1
3,286,910 - +0.3
3,286,971 - +1.4
3,286,971 - +0.7
3,287,005 + +1.4
3,287,018 - +2.5
3,287,018 - +2.1
3,287,020 + +1.1
3,287,026 + +0.6
3,287,026 + +1.0
3,287,026 + +1.1
3,287,027 - +1.4
3,287,027 - +0.9
3,287,027 - +3.0
3,287,028 + +1.1
3,287,029 - +0.9
3,287,120 + +0.7
3,287,137 + +1.0
3,287,320 - BPHYT_RS34255 0.19 -0.9
3,287,320 - BPHYT_RS34255 0.19 -1.0
3,287,352 + BPHYT_RS34255 0.23 -0.5
3,287,403 + BPHYT_RS34255 0.30 +0.1
3,287,404 - BPHYT_RS34255 0.30 -0.4
3,287,510 - BPHYT_RS34255 0.43 +1.5
3,287,512 - BPHYT_RS34255 0.43 -1.2
3,287,512 - BPHYT_RS34255 0.43 +0.1
3,287,855 - BPHYT_RS34255 0.87 +0.4
3,287,871 + BPHYT_RS34255 0.89 +0.2
3,287,872 - BPHYT_RS34255 0.89 -0.7

Or see this region's nucleotide sequence