Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34190

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34180 and BPHYT_RS34185 are separated by 596 nucleotidesBPHYT_RS34185 and BPHYT_RS34190 are separated by 112 nucleotidesBPHYT_RS34190 and BPHYT_RS34195 are separated by 269 nucleotidesBPHYT_RS34195 and BPHYT_RS34200 are separated by 120 nucleotides BPHYT_RS34180: BPHYT_RS34180 - restriction endonuclease, at 3,272,360 to 3,272,683 _RS34180 BPHYT_RS34185: BPHYT_RS34185 - cold-shock protein, at 3,273,280 to 3,273,483 _RS34185 BPHYT_RS34190: BPHYT_RS34190 - chemotaxis protein, at 3,273,596 to 3,275,218 _RS34190 BPHYT_RS34195: BPHYT_RS34195 - thioesterase, at 3,275,488 to 3,275,922 _RS34195 BPHYT_RS34200: BPHYT_RS34200 - ArsR family transcriptional regulator, at 3,276,043 to 3,276,345 _RS34200 Position (kb) 3273 3274 3275 3276Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3272.790 kb on + strandat 3272.791 kb on - strandat 3272.843 kb on - strandat 3272.843 kb on - strandat 3272.843 kb on - strandat 3272.843 kb on - strandat 3272.843 kb on - strandat 3272.843 kb on - strandat 3272.844 kb on + strandat 3272.844 kb on + strandat 3272.844 kb on + strandat 3272.845 kb on - strandat 3272.845 kb on - strandat 3272.845 kb on - strandat 3272.948 kb on - strandat 3272.948 kb on - strandat 3273.052 kb on - strandat 3273.052 kb on - strandat 3273.080 kb on - strandat 3273.080 kb on - strandat 3273.082 kb on + strandat 3273.082 kb on + strandat 3273.082 kb on + strandat 3273.082 kb on + strandat 3273.083 kb on - strandat 3273.158 kb on + strandat 3273.166 kb on + strandat 3273.190 kb on + strandat 3273.201 kb on - strandat 3273.201 kb on - strandat 3273.201 kb on - strandat 3273.201 kb on - strandat 3273.209 kb on + strandat 3273.210 kb on - strandat 3273.238 kb on + strandat 3273.267 kb on + strandat 3273.291 kb on + strandat 3273.292 kb on - strandat 3273.306 kb on + strand, within BPHYT_RS34185at 3273.435 kb on + strand, within BPHYT_RS34185at 3273.436 kb on - strand, within BPHYT_RS34185at 3273.436 kb on - strand, within BPHYT_RS34185at 3273.436 kb on - strand, within BPHYT_RS34185at 3273.481 kb on + strandat 3273.495 kb on + strandat 3273.512 kb on - strandat 3273.525 kb on + strandat 3273.526 kb on - strandat 3274.513 kb on + strand, within BPHYT_RS34190at 3274.513 kb on + strand, within BPHYT_RS34190at 3274.513 kb on + strand, within BPHYT_RS34190at 3274.603 kb on - strand, within BPHYT_RS34190at 3274.730 kb on - strand, within BPHYT_RS34190at 3274.730 kb on - strand, within BPHYT_RS34190at 3274.746 kb on + strand, within BPHYT_RS34190at 3275.175 kb on - strandat 3275.225 kb on + strandat 3275.225 kb on + strandat 3275.253 kb on - strandat 3275.253 kb on - strandat 3275.271 kb on + strandat 3275.417 kb on - strandat 3275.417 kb on - strandat 3275.417 kb on - strandat 3275.549 kb on + strand, within BPHYT_RS34195at 3275.550 kb on - strand, within BPHYT_RS34195at 3275.656 kb on + strand, within BPHYT_RS34195at 3275.657 kb on - strand, within BPHYT_RS34195at 3275.657 kb on - strand, within BPHYT_RS34195at 3275.657 kb on - strand, within BPHYT_RS34195at 3275.725 kb on + strand, within BPHYT_RS34195at 3275.726 kb on - strand, within BPHYT_RS34195at 3275.788 kb on + strand, within BPHYT_RS34195at 3275.827 kb on + strand, within BPHYT_RS34195at 3275.827 kb on + strand, within BPHYT_RS34195at 3275.828 kb on - strand, within BPHYT_RS34195at 3276.183 kb on + strand, within BPHYT_RS34200

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,272,790 + -0.5
3,272,791 - -0.5
3,272,843 - +0.1
3,272,843 - -0.5
3,272,843 - +0.5
3,272,843 - -0.1
3,272,843 - -0.8
3,272,843 - -0.3
3,272,844 + +1.0
3,272,844 + -0.1
3,272,844 + -1.0
3,272,845 - -0.6
3,272,845 - -0.3
3,272,845 - -0.6
3,272,948 - -0.1
3,272,948 - +0.4
3,273,052 - -0.5
3,273,052 - -0.0
3,273,080 - -0.2
3,273,080 - -2.2
3,273,082 + -0.5
3,273,082 + -1.6
3,273,082 + -1.3
3,273,082 + +0.5
3,273,083 - -0.9
3,273,158 + +0.0
3,273,166 + +0.8
3,273,190 + -0.5
3,273,201 - -3.6
3,273,201 - -0.1
3,273,201 - -1.2
3,273,201 - -0.3
3,273,209 + -0.3
3,273,210 - +2.2
3,273,238 + -0.7
3,273,267 + -0.4
3,273,291 + -0.3
3,273,292 - -0.4
3,273,306 + BPHYT_RS34185 0.13 -0.9
3,273,435 + BPHYT_RS34185 0.76 +0.6
3,273,436 - BPHYT_RS34185 0.76 -0.2
3,273,436 - BPHYT_RS34185 0.76 +0.1
3,273,436 - BPHYT_RS34185 0.76 -0.6
3,273,481 + +0.5
3,273,495 + -0.5
3,273,512 - -0.1
3,273,525 + -0.2
3,273,526 - +2.3
3,274,513 + BPHYT_RS34190 0.57 -0.8
3,274,513 + BPHYT_RS34190 0.57 +0.7
3,274,513 + BPHYT_RS34190 0.57 -1.1
3,274,603 - BPHYT_RS34190 0.62 +0.2
3,274,730 - BPHYT_RS34190 0.70 +0.8
3,274,730 - BPHYT_RS34190 0.70 -0.2
3,274,746 + BPHYT_RS34190 0.71 -0.6
3,275,175 - -0.1
3,275,225 + +0.2
3,275,225 + -2.4
3,275,253 - +1.9
3,275,253 - -1.0
3,275,271 + +0.4
3,275,417 - -1.3
3,275,417 - +0.2
3,275,417 - -0.7
3,275,549 + BPHYT_RS34195 0.14 +0.7
3,275,550 - BPHYT_RS34195 0.14 +0.0
3,275,656 + BPHYT_RS34195 0.39 +0.1
3,275,657 - BPHYT_RS34195 0.39 -0.5
3,275,657 - BPHYT_RS34195 0.39 -0.1
3,275,657 - BPHYT_RS34195 0.39 +1.0
3,275,725 + BPHYT_RS34195 0.54 -1.0
3,275,726 - BPHYT_RS34195 0.55 -0.9
3,275,788 + BPHYT_RS34195 0.69 +0.2
3,275,827 + BPHYT_RS34195 0.78 -0.6
3,275,827 + BPHYT_RS34195 0.78 -0.7
3,275,828 - BPHYT_RS34195 0.78 +1.0
3,276,183 + BPHYT_RS34200 0.46 +2.0

Or see this region's nucleotide sequence