Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34180 and BPHYT_RS34185 are separated by 596 nucleotides BPHYT_RS34185 and BPHYT_RS34190 are separated by 112 nucleotides BPHYT_RS34190 and BPHYT_RS34195 are separated by 269 nucleotides BPHYT_RS34195 and BPHYT_RS34200 are separated by 120 nucleotides
BPHYT_RS34180: BPHYT_RS34180 - restriction endonuclease, at 3,272,360 to 3,272,683
_RS34180
BPHYT_RS34185: BPHYT_RS34185 - cold-shock protein, at 3,273,280 to 3,273,483
_RS34185
BPHYT_RS34190: BPHYT_RS34190 - chemotaxis protein, at 3,273,596 to 3,275,218
_RS34190
BPHYT_RS34195: BPHYT_RS34195 - thioesterase, at 3,275,488 to 3,275,922
_RS34195
BPHYT_RS34200: BPHYT_RS34200 - ArsR family transcriptional regulator, at 3,276,043 to 3,276,345
_RS34200
Position (kb)
3273
3274
3275
3276 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3272.790 kb on + strand at 3272.791 kb on - strand at 3272.843 kb on - strand at 3272.843 kb on - strand at 3272.843 kb on - strand at 3272.843 kb on - strand at 3272.843 kb on - strand at 3272.843 kb on - strand at 3272.844 kb on + strand at 3272.844 kb on + strand at 3272.844 kb on + strand at 3272.845 kb on - strand at 3272.845 kb on - strand at 3272.845 kb on - strand at 3272.948 kb on - strand at 3272.948 kb on - strand at 3273.052 kb on - strand at 3273.052 kb on - strand at 3273.080 kb on - strand at 3273.080 kb on - strand at 3273.082 kb on + strand at 3273.082 kb on + strand at 3273.082 kb on + strand at 3273.082 kb on + strand at 3273.083 kb on - strand at 3273.158 kb on + strand at 3273.166 kb on + strand at 3273.190 kb on + strand at 3273.201 kb on - strand at 3273.201 kb on - strand at 3273.201 kb on - strand at 3273.201 kb on - strand at 3273.209 kb on + strand at 3273.210 kb on - strand at 3273.238 kb on + strand at 3273.267 kb on + strand at 3273.291 kb on + strand at 3273.292 kb on - strand at 3273.306 kb on + strand, within BPHYT_RS34185 at 3273.435 kb on + strand, within BPHYT_RS34185 at 3273.436 kb on - strand, within BPHYT_RS34185 at 3273.436 kb on - strand, within BPHYT_RS34185 at 3273.436 kb on - strand, within BPHYT_RS34185 at 3273.481 kb on + strand at 3273.495 kb on + strand at 3273.512 kb on - strand at 3273.525 kb on + strand at 3273.526 kb on - strand at 3274.513 kb on + strand, within BPHYT_RS34190 at 3274.513 kb on + strand, within BPHYT_RS34190 at 3274.513 kb on + strand, within BPHYT_RS34190 at 3274.603 kb on - strand, within BPHYT_RS34190 at 3274.730 kb on - strand, within BPHYT_RS34190 at 3274.730 kb on - strand, within BPHYT_RS34190 at 3274.746 kb on + strand, within BPHYT_RS34190 at 3275.175 kb on - strand at 3275.225 kb on + strand at 3275.225 kb on + strand at 3275.253 kb on - strand at 3275.253 kb on - strand at 3275.271 kb on + strand at 3275.417 kb on - strand at 3275.417 kb on - strand at 3275.417 kb on - strand at 3275.549 kb on + strand, within BPHYT_RS34195 at 3275.550 kb on - strand, within BPHYT_RS34195 at 3275.656 kb on + strand, within BPHYT_RS34195 at 3275.657 kb on - strand, within BPHYT_RS34195 at 3275.657 kb on - strand, within BPHYT_RS34195 at 3275.657 kb on - strand, within BPHYT_RS34195 at 3275.725 kb on + strand, within BPHYT_RS34195 at 3275.726 kb on - strand, within BPHYT_RS34195 at 3275.788 kb on + strand, within BPHYT_RS34195 at 3275.827 kb on + strand, within BPHYT_RS34195 at 3275.827 kb on + strand, within BPHYT_RS34195 at 3275.828 kb on - strand, within BPHYT_RS34195 at 3276.183 kb on + strand, within BPHYT_RS34200
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,272,790 + -0.5 3,272,791 - -0.5 3,272,843 - +0.1 3,272,843 - -0.5 3,272,843 - +0.5 3,272,843 - -0.1 3,272,843 - -0.8 3,272,843 - -0.3 3,272,844 + +1.0 3,272,844 + -0.1 3,272,844 + -1.0 3,272,845 - -0.6 3,272,845 - -0.3 3,272,845 - -0.6 3,272,948 - -0.1 3,272,948 - +0.4 3,273,052 - -0.5 3,273,052 - -0.0 3,273,080 - -0.2 3,273,080 - -2.2 3,273,082 + -0.5 3,273,082 + -1.6 3,273,082 + -1.3 3,273,082 + +0.5 3,273,083 - -0.9 3,273,158 + +0.0 3,273,166 + +0.8 3,273,190 + -0.5 3,273,201 - -3.6 3,273,201 - -0.1 3,273,201 - -1.2 3,273,201 - -0.3 3,273,209 + -0.3 3,273,210 - +2.2 3,273,238 + -0.7 3,273,267 + -0.4 3,273,291 + -0.3 3,273,292 - -0.4 3,273,306 + BPHYT_RS34185 0.13 -0.9 3,273,435 + BPHYT_RS34185 0.76 +0.6 3,273,436 - BPHYT_RS34185 0.76 -0.2 3,273,436 - BPHYT_RS34185 0.76 +0.1 3,273,436 - BPHYT_RS34185 0.76 -0.6 3,273,481 + +0.5 3,273,495 + -0.5 3,273,512 - -0.1 3,273,525 + -0.2 3,273,526 - +2.3 3,274,513 + BPHYT_RS34190 0.57 -0.8 3,274,513 + BPHYT_RS34190 0.57 +0.7 3,274,513 + BPHYT_RS34190 0.57 -1.1 3,274,603 - BPHYT_RS34190 0.62 +0.2 3,274,730 - BPHYT_RS34190 0.70 +0.8 3,274,730 - BPHYT_RS34190 0.70 -0.2 3,274,746 + BPHYT_RS34190 0.71 -0.6 3,275,175 - -0.1 3,275,225 + +0.2 3,275,225 + -2.4 3,275,253 - +1.9 3,275,253 - -1.0 3,275,271 + +0.4 3,275,417 - -1.3 3,275,417 - +0.2 3,275,417 - -0.7 3,275,549 + BPHYT_RS34195 0.14 +0.7 3,275,550 - BPHYT_RS34195 0.14 +0.0 3,275,656 + BPHYT_RS34195 0.39 +0.1 3,275,657 - BPHYT_RS34195 0.39 -0.5 3,275,657 - BPHYT_RS34195 0.39 -0.1 3,275,657 - BPHYT_RS34195 0.39 +1.0 3,275,725 + BPHYT_RS34195 0.54 -1.0 3,275,726 - BPHYT_RS34195 0.55 -0.9 3,275,788 + BPHYT_RS34195 0.69 +0.2 3,275,827 + BPHYT_RS34195 0.78 -0.6 3,275,827 + BPHYT_RS34195 0.78 -0.7 3,275,828 - BPHYT_RS34195 0.78 +1.0 3,276,183 + BPHYT_RS34200 0.46 +2.0
Or see this region's nucleotide sequence