Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33920 and BPHYT_RS33925 are separated by 91 nucleotides BPHYT_RS33925 and BPHYT_RS33930 are separated by 1 nucleotides BPHYT_RS33930 and BPHYT_RS33935 are separated by 24 nucleotides
BPHYT_RS33920: BPHYT_RS33920 - type II citrate synthase, at 3,202,393 to 3,203,694
_RS33920
BPHYT_RS33925: BPHYT_RS33925 - hypothetical protein, at 3,203,786 to 3,204,058
_RS33925
BPHYT_RS33930: BPHYT_RS33930 - succinate dehydrogenase iron-sulfur subunit, at 3,204,060 to 3,204,764
_RS33930
BPHYT_RS33935: BPHYT_RS33935 - succinate dehydrogenase flavoprotein subunit, at 3,204,789 to 3,206,564
_RS33935
Position (kb)
3203
3204
3205 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3202.915 kb on - strand, within BPHYT_RS33920 at 3203.054 kb on + strand, within BPHYT_RS33920 at 3203.055 kb on - strand, within BPHYT_RS33920 at 3203.147 kb on + strand, within BPHYT_RS33920 at 3203.147 kb on + strand, within BPHYT_RS33920 at 3203.147 kb on + strand, within BPHYT_RS33920 at 3203.148 kb on - strand, within BPHYT_RS33920 at 3203.153 kb on + strand, within BPHYT_RS33920 at 3203.154 kb on - strand, within BPHYT_RS33920 at 3203.154 kb on - strand, within BPHYT_RS33920 at 3203.154 kb on - strand, within BPHYT_RS33920 at 3203.246 kb on + strand, within BPHYT_RS33920 at 3203.555 kb on + strand, within BPHYT_RS33920 at 3203.556 kb on - strand, within BPHYT_RS33920 at 3203.579 kb on + strand at 3203.695 kb on - strand at 3203.714 kb on + strand at 3203.715 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,202,915 - BPHYT_RS33920 0.40 -2.5 3,203,054 + BPHYT_RS33920 0.51 -2.7 3,203,055 - BPHYT_RS33920 0.51 -1.6 3,203,147 + BPHYT_RS33920 0.58 -0.9 3,203,147 + BPHYT_RS33920 0.58 +0.2 3,203,147 + BPHYT_RS33920 0.58 -2.5 3,203,148 - BPHYT_RS33920 0.58 -1.1 3,203,153 + BPHYT_RS33920 0.58 -1.5 3,203,154 - BPHYT_RS33920 0.58 -3.2 3,203,154 - BPHYT_RS33920 0.58 -0.8 3,203,154 - BPHYT_RS33920 0.58 -1.6 3,203,246 + BPHYT_RS33920 0.66 -1.8 3,203,555 + BPHYT_RS33920 0.89 -2.3 3,203,556 - BPHYT_RS33920 0.89 -2.3 3,203,579 + -0.6 3,203,695 - -2.6 3,203,714 + -0.1 3,203,715 - -0.9
Or see this region's nucleotide sequence