Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33795 and BPHYT_RS33800 overlap by 4 nucleotides BPHYT_RS33800 and BPHYT_RS33805 are separated by 181 nucleotides BPHYT_RS33805 and BPHYT_RS33810 overlap by 19 nucleotides
BPHYT_RS33795: BPHYT_RS33795 - 4-alpha-glucanotransferase, at 3,172,413 to 3,174,623
_RS33795
BPHYT_RS33800: BPHYT_RS33800 - glycosyl hydrolase, at 3,174,620 to 3,177,475
_RS33800
BPHYT_RS33805: BPHYT_RS33805 - hypothetical protein, at 3,177,657 to 3,177,866
_RS33805
BPHYT_RS33810: BPHYT_RS33810 - membrane protein, at 3,177,848 to 3,178,570
_RS33810
Position (kb)
3174
3175
3176
3177
3178 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3174.490 kb on + strand at 3174.490 kb on + strand at 3174.491 kb on - strand at 3174.699 kb on - strand at 3174.728 kb on + strand at 3174.728 kb on + strand at 3174.774 kb on - strand at 3174.788 kb on + strand at 3174.788 kb on + strand at 3174.789 kb on - strand at 3174.789 kb on - strand at 3174.789 kb on - strand at 3175.030 kb on + strand, within BPHYT_RS33800 at 3175.109 kb on + strand, within BPHYT_RS33800 at 3175.110 kb on - strand, within BPHYT_RS33800 at 3175.154 kb on + strand, within BPHYT_RS33800 at 3175.328 kb on + strand, within BPHYT_RS33800 at 3175.449 kb on + strand, within BPHYT_RS33800 at 3175.449 kb on + strand, within BPHYT_RS33800 at 3175.449 kb on + strand, within BPHYT_RS33800 at 3175.449 kb on + strand, within BPHYT_RS33800 at 3175.450 kb on - strand, within BPHYT_RS33800 at 3175.453 kb on + strand, within BPHYT_RS33800 at 3175.454 kb on - strand, within BPHYT_RS33800 at 3175.589 kb on + strand, within BPHYT_RS33800 at 3175.641 kb on - strand, within BPHYT_RS33800 at 3175.698 kb on + strand, within BPHYT_RS33800 at 3176.001 kb on - strand, within BPHYT_RS33800 at 3176.288 kb on + strand, within BPHYT_RS33800 at 3176.627 kb on + strand, within BPHYT_RS33800 at 3177.153 kb on - strand, within BPHYT_RS33800 at 3177.407 kb on + strand at 3177.510 kb on + strand at 3177.788 kb on - strand, within BPHYT_RS33805 at 3178.114 kb on - strand, within BPHYT_RS33810 at 3178.114 kb on - strand, within BPHYT_RS33810 at 3178.356 kb on + strand, within BPHYT_RS33810
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,174,490 + +0.1 3,174,490 + -0.3 3,174,491 - -1.1 3,174,699 - +0.7 3,174,728 + +0.1 3,174,728 + -1.2 3,174,774 - -3.0 3,174,788 + -1.2 3,174,788 + -0.2 3,174,789 - -1.0 3,174,789 - -0.5 3,174,789 - -1.0 3,175,030 + BPHYT_RS33800 0.14 +1.4 3,175,109 + BPHYT_RS33800 0.17 -0.9 3,175,110 - BPHYT_RS33800 0.17 -1.4 3,175,154 + BPHYT_RS33800 0.19 -0.4 3,175,328 + BPHYT_RS33800 0.25 -0.9 3,175,449 + BPHYT_RS33800 0.29 +0.1 3,175,449 + BPHYT_RS33800 0.29 -2.3 3,175,449 + BPHYT_RS33800 0.29 -0.7 3,175,449 + BPHYT_RS33800 0.29 -1.1 3,175,450 - BPHYT_RS33800 0.29 +0.4 3,175,453 + BPHYT_RS33800 0.29 -0.5 3,175,454 - BPHYT_RS33800 0.29 -1.3 3,175,589 + BPHYT_RS33800 0.34 -1.2 3,175,641 - BPHYT_RS33800 0.36 +0.7 3,175,698 + BPHYT_RS33800 0.38 -3.7 3,176,001 - BPHYT_RS33800 0.48 -0.8 3,176,288 + BPHYT_RS33800 0.58 -0.8 3,176,627 + BPHYT_RS33800 0.70 -0.1 3,177,153 - BPHYT_RS33800 0.89 -0.5 3,177,407 + -1.0 3,177,510 + +0.1 3,177,788 - BPHYT_RS33805 0.62 -0.0 3,178,114 - BPHYT_RS33810 0.37 +0.4 3,178,114 - BPHYT_RS33810 0.37 -0.4 3,178,356 + BPHYT_RS33810 0.70 +1.3
Or see this region's nucleotide sequence