Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33785 and BPHYT_RS33790 are separated by 140 nucleotides BPHYT_RS33790 and BPHYT_RS33795 overlap by 4 nucleotides
BPHYT_RS33785: BPHYT_RS33785 - glycogen debranching protein, at 3,168,134 to 3,170,353
_RS33785
BPHYT_RS33790: BPHYT_RS33790 - malto-oligosyltrehalose trehalohydrolase, at 3,170,494 to 3,172,416
_RS33790
BPHYT_RS33795: BPHYT_RS33795 - 4-alpha-glucanotransferase, at 3,172,413 to 3,174,623
_RS33795
Position (kb)
3170
3171
3172
3173 Strain fitness (log2 ratio)
-1
0
1
2 at 3170.052 kb on + strand, within BPHYT_RS33785 at 3170.052 kb on + strand, within BPHYT_RS33785 at 3170.052 kb on + strand, within BPHYT_RS33785 at 3170.052 kb on + strand, within BPHYT_RS33785 at 3170.134 kb on - strand at 3170.200 kb on + strand at 3170.428 kb on + strand at 3170.564 kb on + strand at 3170.847 kb on + strand, within BPHYT_RS33790 at 3170.888 kb on - strand, within BPHYT_RS33790 at 3170.918 kb on - strand, within BPHYT_RS33790 at 3171.134 kb on - strand, within BPHYT_RS33790 at 3171.160 kb on + strand, within BPHYT_RS33790 at 3171.520 kb on + strand, within BPHYT_RS33790 at 3171.523 kb on + strand, within BPHYT_RS33790 at 3171.523 kb on + strand, within BPHYT_RS33790 at 3171.524 kb on - strand, within BPHYT_RS33790 at 3171.533 kb on - strand, within BPHYT_RS33790 at 3171.811 kb on + strand, within BPHYT_RS33790 at 3171.847 kb on + strand, within BPHYT_RS33790 at 3172.107 kb on + strand, within BPHYT_RS33790 at 3172.324 kb on + strand at 3172.807 kb on + strand, within BPHYT_RS33795 at 3172.854 kb on + strand, within BPHYT_RS33795 at 3172.856 kb on + strand, within BPHYT_RS33795
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,170,052 + BPHYT_RS33785 0.86 +1.8 3,170,052 + BPHYT_RS33785 0.86 -0.2 3,170,052 + BPHYT_RS33785 0.86 -0.2 3,170,052 + BPHYT_RS33785 0.86 -0.2 3,170,134 - -0.4 3,170,200 + +0.3 3,170,428 + +1.9 3,170,564 + +0.0 3,170,847 + BPHYT_RS33790 0.18 +0.2 3,170,888 - BPHYT_RS33790 0.20 -0.6 3,170,918 - BPHYT_RS33790 0.22 -0.7 3,171,134 - BPHYT_RS33790 0.33 +0.5 3,171,160 + BPHYT_RS33790 0.35 +1.1 3,171,520 + BPHYT_RS33790 0.53 -0.4 3,171,523 + BPHYT_RS33790 0.54 -1.6 3,171,523 + BPHYT_RS33790 0.54 +0.4 3,171,524 - BPHYT_RS33790 0.54 -0.3 3,171,533 - BPHYT_RS33790 0.54 -0.5 3,171,811 + BPHYT_RS33790 0.68 -0.1 3,171,847 + BPHYT_RS33790 0.70 +2.6 3,172,107 + BPHYT_RS33790 0.84 -0.0 3,172,324 + -1.4 3,172,807 + BPHYT_RS33795 0.18 +0.1 3,172,854 + BPHYT_RS33795 0.20 -0.1 3,172,856 + BPHYT_RS33795 0.20 -0.8
Or see this region's nucleotide sequence