Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33625 and BPHYT_RS33630 overlap by 4 nucleotides BPHYT_RS33630 and BPHYT_RS33635 are separated by 58 nucleotides BPHYT_RS33635 and BPHYT_RS33640 are separated by 199 nucleotides BPHYT_RS33640 and BPHYT_RS33645 are separated by 223 nucleotides
BPHYT_RS33625: BPHYT_RS33625 - chromate transporter, at 3,129,647 to 3,130,216
_RS33625
BPHYT_RS33630: BPHYT_RS33630 - chromate transporter, at 3,130,213 to 3,130,743
_RS33630
BPHYT_RS33635: BPHYT_RS33635 - AMP nucleosidase, at 3,130,802 to 3,132,328
_RS33635
BPHYT_RS33640: BPHYT_RS33640 - hypothetical protein, at 3,132,528 to 3,132,923
_RS33640
BPHYT_RS33645: BPHYT_RS33645 - homoserine kinase, at 3,133,147 to 3,134,142
_RS33645
Position (kb)
3130
3131
3132
3133 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3130.183 kb on + strand at 3130.183 kb on + strand at 3130.183 kb on + strand at 3130.183 kb on + strand at 3130.184 kb on - strand at 3130.190 kb on + strand at 3130.190 kb on + strand at 3130.190 kb on + strand at 3130.191 kb on - strand at 3130.191 kb on - strand at 3130.243 kb on - strand at 3130.284 kb on + strand, within BPHYT_RS33630 at 3130.285 kb on - strand, within BPHYT_RS33630 at 3130.803 kb on + strand at 3130.804 kb on - strand at 3130.875 kb on - strand at 3131.001 kb on + strand, within BPHYT_RS33635 at 3131.002 kb on - strand, within BPHYT_RS33635 at 3131.310 kb on + strand, within BPHYT_RS33635 at 3131.311 kb on - strand, within BPHYT_RS33635 at 3131.476 kb on - strand, within BPHYT_RS33635 at 3131.544 kb on + strand, within BPHYT_RS33635 at 3131.545 kb on - strand, within BPHYT_RS33635 at 3131.545 kb on - strand, within BPHYT_RS33635 at 3131.581 kb on - strand, within BPHYT_RS33635 at 3131.659 kb on + strand, within BPHYT_RS33635 at 3131.662 kb on - strand, within BPHYT_RS33635 at 3131.670 kb on + strand, within BPHYT_RS33635 at 3131.670 kb on + strand, within BPHYT_RS33635 at 3131.679 kb on + strand, within BPHYT_RS33635 at 3131.679 kb on + strand, within BPHYT_RS33635 at 3131.680 kb on - strand, within BPHYT_RS33635 at 3131.688 kb on + strand, within BPHYT_RS33635 at 3131.722 kb on - strand, within BPHYT_RS33635 at 3131.722 kb on - strand, within BPHYT_RS33635 at 3131.806 kb on - strand, within BPHYT_RS33635 at 3131.806 kb on - strand, within BPHYT_RS33635 at 3131.806 kb on - strand, within BPHYT_RS33635 at 3131.806 kb on - strand, within BPHYT_RS33635 at 3131.806 kb on - strand, within BPHYT_RS33635 at 3132.015 kb on + strand, within BPHYT_RS33635 at 3132.015 kb on + strand, within BPHYT_RS33635 at 3132.016 kb on - strand, within BPHYT_RS33635 at 3132.016 kb on - strand, within BPHYT_RS33635 at 3132.019 kb on + strand, within BPHYT_RS33635 at 3132.101 kb on - strand, within BPHYT_RS33635 at 3132.138 kb on + strand, within BPHYT_RS33635 at 3132.317 kb on - strand at 3132.342 kb on + strand at 3132.342 kb on + strand at 3132.360 kb on - strand at 3132.461 kb on + strand at 3132.513 kb on + strand at 3132.513 kb on + strand at 3132.513 kb on + strand at 3132.738 kb on + strand, within BPHYT_RS33640 at 3132.918 kb on + strand at 3133.202 kb on - strand at 3133.288 kb on + strand, within BPHYT_RS33645 at 3133.288 kb on + strand, within BPHYT_RS33645
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,130,183 + -0.1 3,130,183 + -0.4 3,130,183 + -1.0 3,130,183 + +0.9 3,130,184 - -1.0 3,130,190 + -0.3 3,130,190 + +0.0 3,130,190 + -0.1 3,130,191 - -0.1 3,130,191 - +3.3 3,130,243 - +2.4 3,130,284 + BPHYT_RS33630 0.13 -1.6 3,130,285 - BPHYT_RS33630 0.14 -1.1 3,130,803 + +0.5 3,130,804 - +0.1 3,130,875 - -1.0 3,131,001 + BPHYT_RS33635 0.13 -0.9 3,131,002 - BPHYT_RS33635 0.13 -3.1 3,131,310 + BPHYT_RS33635 0.33 -2.8 3,131,311 - BPHYT_RS33635 0.33 -2.3 3,131,476 - BPHYT_RS33635 0.44 -0.9 3,131,544 + BPHYT_RS33635 0.49 -0.8 3,131,545 - BPHYT_RS33635 0.49 -2.8 3,131,545 - BPHYT_RS33635 0.49 -2.0 3,131,581 - BPHYT_RS33635 0.51 -2.4 3,131,659 + BPHYT_RS33635 0.56 -1.9 3,131,662 - BPHYT_RS33635 0.56 -3.3 3,131,670 + BPHYT_RS33635 0.57 -2.3 3,131,670 + BPHYT_RS33635 0.57 -2.0 3,131,679 + BPHYT_RS33635 0.57 -3.1 3,131,679 + BPHYT_RS33635 0.57 -1.5 3,131,680 - BPHYT_RS33635 0.57 -2.1 3,131,688 + BPHYT_RS33635 0.58 -1.9 3,131,722 - BPHYT_RS33635 0.60 -2.9 3,131,722 - BPHYT_RS33635 0.60 -1.6 3,131,806 - BPHYT_RS33635 0.66 -1.0 3,131,806 - BPHYT_RS33635 0.66 -1.7 3,131,806 - BPHYT_RS33635 0.66 -0.6 3,131,806 - BPHYT_RS33635 0.66 -1.7 3,131,806 - BPHYT_RS33635 0.66 -0.3 3,132,015 + BPHYT_RS33635 0.79 -2.9 3,132,015 + BPHYT_RS33635 0.79 -2.5 3,132,016 - BPHYT_RS33635 0.80 -2.8 3,132,016 - BPHYT_RS33635 0.80 -3.3 3,132,019 + BPHYT_RS33635 0.80 -2.2 3,132,101 - BPHYT_RS33635 0.85 -1.1 3,132,138 + BPHYT_RS33635 0.87 -1.5 3,132,317 - -0.6 3,132,342 + -0.3 3,132,342 + -1.7 3,132,360 - -0.4 3,132,461 + +0.8 3,132,513 + -0.5 3,132,513 + -0.6 3,132,513 + -0.3 3,132,738 + BPHYT_RS33640 0.53 +1.8 3,132,918 + +3.2 3,133,202 - +2.3 3,133,288 + BPHYT_RS33645 0.14 -4.2 3,133,288 + BPHYT_RS33645 0.14 -0.7
Or see this region's nucleotide sequence