Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33225

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33220 and BPHYT_RS33225 are separated by 472 nucleotides BPHYT_RS33220: BPHYT_RS33220 - group 1 glycosyl transferase, at 3,030,646 to 3,032,643 _RS33220 BPHYT_RS33225: BPHYT_RS33225 - NAD-dependent epimerase/dehydratase, at 3,033,116 to 3,034,060 _RS33225 Position (kb) 3033 3034 3035Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3032.117 kb on + strand, within BPHYT_RS33220at 3032.129 kb on + strand, within BPHYT_RS33220at 3032.130 kb on - strand, within BPHYT_RS33220at 3032.133 kb on - strand, within BPHYT_RS33220at 3032.133 kb on - strand, within BPHYT_RS33220at 3032.269 kb on - strand, within BPHYT_RS33220at 3032.628 kb on + strandat 3032.664 kb on + strandat 3032.720 kb on - strandat 3033.075 kb on + strandat 3033.151 kb on - strandat 3033.151 kb on - strandat 3033.332 kb on + strand, within BPHYT_RS33225at 3033.575 kb on - strand, within BPHYT_RS33225at 3033.606 kb on + strand, within BPHYT_RS33225at 3033.614 kb on + strand, within BPHYT_RS33225at 3033.619 kb on - strand, within BPHYT_RS33225at 3033.656 kb on + strand, within BPHYT_RS33225at 3033.657 kb on - strand, within BPHYT_RS33225at 3033.773 kb on + strand, within BPHYT_RS33225at 3033.796 kb on + strand, within BPHYT_RS33225at 3034.065 kb on + strandat 3034.111 kb on - strandat 3034.127 kb on - strandat 3034.194 kb on - strandat 3034.225 kb on - strandat 3034.225 kb on - strandat 3034.258 kb on - strandat 3034.397 kb on - strandat 3034.409 kb on + strandat 3034.457 kb on - strandat 3034.463 kb on + strandat 3034.695 kb on - strandat 3034.756 kb on - strandat 3034.828 kb on - strandat 3034.877 kb on + strandat 3034.878 kb on - strandat 3034.997 kb on + strandat 3034.997 kb on + strandat 3034.998 kb on - strandat 3034.998 kb on - strandat 3034.998 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,032,117 + BPHYT_RS33220 0.74 +2.3
3,032,129 + BPHYT_RS33220 0.74 +2.8
3,032,130 - BPHYT_RS33220 0.74 -0.7
3,032,133 - BPHYT_RS33220 0.74 +0.1
3,032,133 - BPHYT_RS33220 0.74 -1.4
3,032,269 - BPHYT_RS33220 0.81 +1.3
3,032,628 + +1.8
3,032,664 + +2.2
3,032,720 - +0.4
3,033,075 + +0.0
3,033,151 - -1.1
3,033,151 - +2.0
3,033,332 + BPHYT_RS33225 0.23 -0.4
3,033,575 - BPHYT_RS33225 0.49 +4.1
3,033,606 + BPHYT_RS33225 0.52 +0.6
3,033,614 + BPHYT_RS33225 0.53 +0.6
3,033,619 - BPHYT_RS33225 0.53 +0.2
3,033,656 + BPHYT_RS33225 0.57 +4.2
3,033,657 - BPHYT_RS33225 0.57 -0.1
3,033,773 + BPHYT_RS33225 0.70 +0.9
3,033,796 + BPHYT_RS33225 0.72 -0.3
3,034,065 + +0.3
3,034,111 - +0.9
3,034,127 - -0.1
3,034,194 - +1.0
3,034,225 - -0.1
3,034,225 - +2.0
3,034,258 - +0.2
3,034,397 - +0.5
3,034,409 + +1.8
3,034,457 - -1.5
3,034,463 + -0.7
3,034,695 - +0.2
3,034,756 - -0.9
3,034,828 - +1.8
3,034,877 + -2.4
3,034,878 - -2.3
3,034,997 + +0.8
3,034,997 + -0.5
3,034,998 - +1.3
3,034,998 - +1.6
3,034,998 - +0.7

Or see this region's nucleotide sequence