Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32955

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBPHYT_RS32950 and BPHYT_RS32955 are separated by 468 nucleotidesBPHYT_RS32955 and BPHYT_RS32960 are separated by 13 nucleotidesBPHYT_RS32960 and BPHYT_RS32965 are separated by 92 nucleotides BPHYT_RS32950: BPHYT_RS32950 - transposase, at 2,971,779 to 2,972,948 _RS32950 BPHYT_RS32955: BPHYT_RS32955 - polyketide synthase, at 2,973,417 to 2,974,073 _RS32955 BPHYT_RS32960: BPHYT_RS32960 - short-chain dehydrogenase, at 2,974,087 to 2,974,812 _RS32960 BPHYT_RS32965: BPHYT_RS32965 - HxlR family transcriptional regulator, at 2,974,905 to 2,975,300 _RS32965 Position (kb) 2973 2974 2975Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2973.291 kb on + strandat 2973.355 kb on + strandat 2973.383 kb on + strandat 2973.383 kb on + strandat 2973.383 kb on + strandat 2973.384 kb on - strandat 2973.418 kb on + strandat 2973.419 kb on - strandat 2973.419 kb on - strandat 2973.429 kb on + strandat 2973.491 kb on + strand, within BPHYT_RS32955at 2973.703 kb on + strand, within BPHYT_RS32955at 2973.741 kb on - strand, within BPHYT_RS32955at 2973.822 kb on + strand, within BPHYT_RS32955at 2973.914 kb on - strand, within BPHYT_RS32955at 2973.914 kb on - strand, within BPHYT_RS32955at 2974.029 kb on - strandat 2974.053 kb on - strandat 2974.073 kb on + strandat 2974.074 kb on - strandat 2974.320 kb on - strand, within BPHYT_RS32960at 2974.320 kb on - strand, within BPHYT_RS32960at 2974.833 kb on + strandat 2974.834 kb on - strandat 2974.852 kb on - strandat 2974.852 kb on - strandat 2974.861 kb on - strandat 2974.886 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
remove
2,973,291 + +0.6
2,973,355 + -0.6
2,973,383 + -0.8
2,973,383 + -0.4
2,973,383 + +0.0
2,973,384 - -0.7
2,973,418 + -0.5
2,973,419 - -0.5
2,973,419 - +0.1
2,973,429 + +4.4
2,973,491 + BPHYT_RS32955 0.11 +1.5
2,973,703 + BPHYT_RS32955 0.44 -0.7
2,973,741 - BPHYT_RS32955 0.49 -2.3
2,973,822 + BPHYT_RS32955 0.62 +1.8
2,973,914 - BPHYT_RS32955 0.76 +0.4
2,973,914 - BPHYT_RS32955 0.76 -0.9
2,974,029 - +2.6
2,974,053 - +1.0
2,974,073 + -0.5
2,974,074 - +1.4
2,974,320 - BPHYT_RS32960 0.32 +1.5
2,974,320 - BPHYT_RS32960 0.32 -1.0
2,974,833 + +0.3
2,974,834 - -0.8
2,974,852 - -0.4
2,974,852 - -1.3
2,974,861 - +0.5
2,974,886 - +1.3

Or see this region's nucleotide sequence