Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32840 and BPHYT_RS32845 are separated by 729 nucleotides BPHYT_RS32845 and BPHYT_RS32850 are separated by 118 nucleotides BPHYT_RS32850 and BPHYT_RS32855 are separated by 109 nucleotides
BPHYT_RS32840: BPHYT_RS32840 - hypothetical protein, at 2,949,239 to 2,949,541
_RS32840
BPHYT_RS32845: BPHYT_RS32845 - ABC transporter substrate-binding protein, at 2,950,271 to 2,951,389
_RS32845
BPHYT_RS32850: BPHYT_RS32850 - transcriptional regulator, at 2,951,508 to 2,951,870
_RS32850
BPHYT_RS32855: BPHYT_RS32855 - TetR family transcriptional regulator, at 2,951,980 to 2,952,474
_RS32855
Position (kb)
2950
2951
2952 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2949.675 kb on - strand at 2949.873 kb on - strand at 2950.124 kb on + strand at 2950.454 kb on + strand, within BPHYT_RS32845 at 2950.463 kb on + strand, within BPHYT_RS32845 at 2950.864 kb on + strand, within BPHYT_RS32845 at 2951.169 kb on - strand at 2951.200 kb on + strand, within BPHYT_RS32845 at 2951.522 kb on + strand at 2951.555 kb on + strand, within BPHYT_RS32850 at 2951.706 kb on + strand, within BPHYT_RS32850
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,949,675 - -0.4 2,949,873 - +1.8 2,950,124 + -0.7 2,950,454 + BPHYT_RS32845 0.16 +0.4 2,950,463 + BPHYT_RS32845 0.17 +0.7 2,950,864 + BPHYT_RS32845 0.53 +1.1 2,951,169 - -0.1 2,951,200 + BPHYT_RS32845 0.83 -2.3 2,951,522 + +0.2 2,951,555 + BPHYT_RS32850 0.13 +0.1 2,951,706 + BPHYT_RS32850 0.55 -0.2
Or see this region's nucleotide sequence