Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32535

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32530 and BPHYT_RS32535 are separated by 33 nucleotidesBPHYT_RS32535 and BPHYT_RS32540 are separated by 187 nucleotidesBPHYT_RS32540 and BPHYT_RS32545 are separated by 152 nucleotidesBPHYT_RS32545 and BPHYT_RS32550 are separated by 1 nucleotides BPHYT_RS32530: BPHYT_RS32530 - transposase, at 2,869,936 to 2,871,492 _RS32530 BPHYT_RS32535: BPHYT_RS32535 - transposase, at 2,871,526 to 2,871,876 _RS32535 BPHYT_RS32540: BPHYT_RS32540 - hypothetical protein, at 2,872,064 to 2,872,321 _RS32540 BPHYT_RS32545: BPHYT_RS32545 - hypothetical protein, at 2,872,474 to 2,872,689 _RS32545 BPHYT_RS32550: BPHYT_RS32550 - general secretion pathway protein A, at 2,872,691 to 2,873,548 _RS32550 Position (kb) 2871 2872Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2870.558 kb on + strand, within BPHYT_RS32530at 2870.575 kb on + strand, within BPHYT_RS32530at 2870.640 kb on - strand, within BPHYT_RS32530at 2870.690 kb on + strand, within BPHYT_RS32530at 2870.690 kb on + strand, within BPHYT_RS32530at 2870.919 kb on + strand, within BPHYT_RS32530at 2870.919 kb on + strand, within BPHYT_RS32530at 2871.366 kb on - strandat 2871.502 kb on - strandat 2871.592 kb on + strand, within BPHYT_RS32535at 2871.646 kb on + strand, within BPHYT_RS32535at 2871.646 kb on + strand, within BPHYT_RS32535at 2871.746 kb on + strand, within BPHYT_RS32535at 2871.845 kb on + strandat 2871.845 kb on + strandat 2871.846 kb on - strandat 2871.846 kb on - strandat 2872.082 kb on + strandat 2872.267 kb on - strand, within BPHYT_RS32540at 2872.370 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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2,870,558 + BPHYT_RS32530 0.40 -0.4
2,870,575 + BPHYT_RS32530 0.41 -1.1
2,870,640 - BPHYT_RS32530 0.45 +0.6
2,870,690 + BPHYT_RS32530 0.48 -0.6
2,870,690 + BPHYT_RS32530 0.48 +2.8
2,870,919 + BPHYT_RS32530 0.63 -0.5
2,870,919 + BPHYT_RS32530 0.63 -1.5
2,871,366 - +0.9
2,871,502 - +0.1
2,871,592 + BPHYT_RS32535 0.19 -0.0
2,871,646 + BPHYT_RS32535 0.34 -0.6
2,871,646 + BPHYT_RS32535 0.34 -0.3
2,871,746 + BPHYT_RS32535 0.63 -0.4
2,871,845 + +0.2
2,871,845 + -0.0
2,871,846 - +0.2
2,871,846 - -0.5
2,872,082 + -2.4
2,872,267 - BPHYT_RS32540 0.79 -1.1
2,872,370 + +0.9

Or see this region's nucleotide sequence