Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32505 and BPHYT_RS32510 overlap by 4 nucleotides BPHYT_RS32510 and BPHYT_RS32515 are separated by 83 nucleotides BPHYT_RS32515 and BPHYT_RS32520 are separated by 275 nucleotides
BPHYT_RS32505: BPHYT_RS32505 - ABC transporter permease, at 2,864,225 to 2,865,280
_RS32505
BPHYT_RS32510: BPHYT_RS32510 - ABC transporter permease, at 2,865,277 to 2,866,137
_RS32510
BPHYT_RS32515: BPHYT_RS32515 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,866,221 to 2,867,429
_RS32515
BPHYT_RS32520: BPHYT_RS32520 - GntR family transcriptional regulator, at 2,867,705 to 2,868,553
_RS32520
Position (kb)
2866
2867
2868 Strain fitness (log2 ratio)
-1
0
1 at 2865.338 kb on + strand at 2865.879 kb on - strand, within BPHYT_RS32510 at 2866.085 kb on + strand at 2866.490 kb on - strand, within BPHYT_RS32515 at 2866.508 kb on - strand, within BPHYT_RS32515 at 2866.752 kb on + strand, within BPHYT_RS32515 at 2866.830 kb on + strand, within BPHYT_RS32515 at 2866.897 kb on + strand, within BPHYT_RS32515 at 2866.898 kb on - strand, within BPHYT_RS32515 at 2867.209 kb on + strand, within BPHYT_RS32515 at 2867.549 kb on - strand at 2867.560 kb on + strand at 2867.977 kb on + strand, within BPHYT_RS32520 at 2868.036 kb on - strand, within BPHYT_RS32520 at 2868.247 kb on + strand, within BPHYT_RS32520
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,865,338 + +1.5 2,865,879 - BPHYT_RS32510 0.70 -1.0 2,866,085 + +0.6 2,866,490 - BPHYT_RS32515 0.22 -0.2 2,866,508 - BPHYT_RS32515 0.24 +0.6 2,866,752 + BPHYT_RS32515 0.44 +1.6 2,866,830 + BPHYT_RS32515 0.50 -0.1 2,866,897 + BPHYT_RS32515 0.56 -0.4 2,866,898 - BPHYT_RS32515 0.56 -0.0 2,867,209 + BPHYT_RS32515 0.82 -0.5 2,867,549 - -0.9 2,867,560 + +0.1 2,867,977 + BPHYT_RS32520 0.32 +0.6 2,868,036 - BPHYT_RS32520 0.39 -0.5 2,868,247 + BPHYT_RS32520 0.64 -1.3
Or see this region's nucleotide sequence