Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32515

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32505 and BPHYT_RS32510 overlap by 4 nucleotidesBPHYT_RS32510 and BPHYT_RS32515 are separated by 83 nucleotidesBPHYT_RS32515 and BPHYT_RS32520 are separated by 275 nucleotides BPHYT_RS32505: BPHYT_RS32505 - ABC transporter permease, at 2,864,225 to 2,865,280 _RS32505 BPHYT_RS32510: BPHYT_RS32510 - ABC transporter permease, at 2,865,277 to 2,866,137 _RS32510 BPHYT_RS32515: BPHYT_RS32515 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,866,221 to 2,867,429 _RS32515 BPHYT_RS32520: BPHYT_RS32520 - GntR family transcriptional regulator, at 2,867,705 to 2,868,553 _RS32520 Position (kb) 2866 2867 2868Strain fitness (log2 ratio) -1 0 1at 2865.338 kb on + strandat 2865.879 kb on - strand, within BPHYT_RS32510at 2866.085 kb on + strandat 2866.490 kb on - strand, within BPHYT_RS32515at 2866.508 kb on - strand, within BPHYT_RS32515at 2866.752 kb on + strand, within BPHYT_RS32515at 2866.830 kb on + strand, within BPHYT_RS32515at 2866.897 kb on + strand, within BPHYT_RS32515at 2866.898 kb on - strand, within BPHYT_RS32515at 2867.209 kb on + strand, within BPHYT_RS32515at 2867.549 kb on - strandat 2867.560 kb on + strandat 2867.977 kb on + strand, within BPHYT_RS32520at 2868.036 kb on - strand, within BPHYT_RS32520at 2868.247 kb on + strand, within BPHYT_RS32520

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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2,865,338 + +1.5
2,865,879 - BPHYT_RS32510 0.70 -1.0
2,866,085 + +0.6
2,866,490 - BPHYT_RS32515 0.22 -0.2
2,866,508 - BPHYT_RS32515 0.24 +0.6
2,866,752 + BPHYT_RS32515 0.44 +1.6
2,866,830 + BPHYT_RS32515 0.50 -0.1
2,866,897 + BPHYT_RS32515 0.56 -0.4
2,866,898 - BPHYT_RS32515 0.56 -0.0
2,867,209 + BPHYT_RS32515 0.82 -0.5
2,867,549 - -0.9
2,867,560 + +0.1
2,867,977 + BPHYT_RS32520 0.32 +0.6
2,868,036 - BPHYT_RS32520 0.39 -0.5
2,868,247 + BPHYT_RS32520 0.64 -1.3

Or see this region's nucleotide sequence