Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32290 and BPHYT_RS32295 are separated by 86 nucleotides BPHYT_RS32295 and BPHYT_RS32300 are separated by 6 nucleotides BPHYT_RS32300 and BPHYT_RS32305 are separated by 202 nucleotides BPHYT_RS32305 and BPHYT_RS32310 are separated by 154 nucleotides
BPHYT_RS32290: BPHYT_RS32290 - hypothetical protein, at 2,814,521 to 2,815,210
_RS32290
BPHYT_RS32295: BPHYT_RS32295 - ATP-binding protein, at 2,815,297 to 2,816,085
_RS32295
BPHYT_RS32300: BPHYT_RS32300 - integrase, at 2,816,092 to 2,817,609
_RS32300
BPHYT_RS32305: BPHYT_RS32305 - hypothetical protein, at 2,817,812 to 2,818,234
_RS32305
BPHYT_RS32310: BPHYT_RS32310 - porin, at 2,818,389 to 2,819,576
_RS32310
Position (kb)
2816
2817
2818 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2815.094 kb on + strand, within BPHYT_RS32290 at 2817.813 kb on + strand at 2817.848 kb on + strand at 2817.860 kb on + strand, within BPHYT_RS32305 at 2817.948 kb on + strand, within BPHYT_RS32305 at 2818.328 kb on - strand at 2818.328 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,815,094 + BPHYT_RS32290 0.83 +3.0 2,817,813 + -0.3 2,817,848 + -0.1 2,817,860 + BPHYT_RS32305 0.11 -1.6 2,817,948 + BPHYT_RS32305 0.32 +1.9 2,818,328 - -0.5 2,818,328 - +1.0
Or see this region's nucleotide sequence