Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32215 and BPHYT_RS32220 are separated by 116 nucleotides BPHYT_RS32220 and BPHYT_RS32225 are separated by 237 nucleotides BPHYT_RS32225 and BPHYT_RS32230 are separated by 214 nucleotides
BPHYT_RS32215: BPHYT_RS32215 - AlpA family transcriptional regulator, at 2,798,277 to 2,798,510
_RS32215
BPHYT_RS32220: BPHYT_RS32220 - hypothetical protein, at 2,798,627 to 2,799,163
_RS32220
BPHYT_RS32225: BPHYT_RS32225 - hypothetical protein, at 2,799,401 to 2,799,799
_RS32225
BPHYT_RS32230: BPHYT_RS32230 - fatty acid desaturase, at 2,800,014 to 2,800,958
_RS32230
Position (kb)
2799
2800 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2799.346 kb on + strand at 2799.512 kb on - strand, within BPHYT_RS32225 at 2799.730 kb on - strand, within BPHYT_RS32225 at 2799.736 kb on - strand, within BPHYT_RS32225 at 2799.828 kb on + strand at 2799.893 kb on + strand at 2799.957 kb on + strand at 2799.958 kb on - strand at 2800.073 kb on - strand at 2800.157 kb on - strand, within BPHYT_RS32230 at 2800.157 kb on - strand, within BPHYT_RS32230 at 2800.186 kb on + strand, within BPHYT_RS32230 at 2800.203 kb on - strand, within BPHYT_RS32230 at 2800.479 kb on - strand, within BPHYT_RS32230 at 2800.729 kb on - strand, within BPHYT_RS32230
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,799,346 + -0.6 2,799,512 - BPHYT_RS32225 0.28 +3.1 2,799,730 - BPHYT_RS32225 0.82 -1.9 2,799,736 - BPHYT_RS32225 0.84 -0.7 2,799,828 + +0.4 2,799,893 + +0.5 2,799,957 + +0.3 2,799,958 - -1.0 2,800,073 - -1.4 2,800,157 - BPHYT_RS32230 0.15 -0.8 2,800,157 - BPHYT_RS32230 0.15 -0.9 2,800,186 + BPHYT_RS32230 0.18 -0.5 2,800,203 - BPHYT_RS32230 0.20 -0.8 2,800,479 - BPHYT_RS32230 0.49 +0.6 2,800,729 - BPHYT_RS32230 0.76 +0.0
Or see this region's nucleotide sequence