Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32140 and BPHYT_RS32145 overlap by 4 nucleotides BPHYT_RS32145 and BPHYT_RS32150 overlap by 4 nucleotides BPHYT_RS32150 and BPHYT_RS32155 are separated by 7 nucleotides
BPHYT_RS32140: BPHYT_RS32140 - DNA replication protein, at 2,783,281 to 2,785,146
_RS32140
BPHYT_RS32145: BPHYT_RS32145 - type IV secretory pathway protein VirD4, at 2,785,143 to 2,786,894
_RS32145
BPHYT_RS32150: BPHYT_RS32150 - hypothetical protein, at 2,786,891 to 2,787,295
_RS32150
BPHYT_RS32155: BPHYT_RS32155 - type IV secretion system protein VirB11, at 2,787,303 to 2,788,421
_RS32155
Position (kb)
2785
2786
2787 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2784.629 kb on + strand, within BPHYT_RS32140 at 2784.630 kb on - strand, within BPHYT_RS32140 at 2784.708 kb on - strand, within BPHYT_RS32140 at 2785.140 kb on + strand at 2785.140 kb on + strand at 2785.140 kb on + strand at 2785.141 kb on - strand at 2785.141 kb on - strand at 2785.328 kb on - strand, within BPHYT_RS32145 at 2785.408 kb on + strand, within BPHYT_RS32145 at 2785.409 kb on - strand, within BPHYT_RS32145 at 2785.585 kb on + strand, within BPHYT_RS32145 at 2785.826 kb on - strand, within BPHYT_RS32145 at 2785.859 kb on + strand, within BPHYT_RS32145 at 2785.859 kb on + strand, within BPHYT_RS32145 at 2785.946 kb on - strand, within BPHYT_RS32145 at 2786.135 kb on - strand, within BPHYT_RS32145 at 2786.414 kb on - strand, within BPHYT_RS32145 at 2786.581 kb on - strand, within BPHYT_RS32145 at 2786.740 kb on + strand at 2786.847 kb on + strand at 2786.946 kb on + strand, within BPHYT_RS32150 at 2787.006 kb on - strand, within BPHYT_RS32150 at 2787.147 kb on + strand, within BPHYT_RS32150 at 2787.710 kb on - strand, within BPHYT_RS32155 at 2787.816 kb on - strand, within BPHYT_RS32155 at 2787.816 kb on - strand, within BPHYT_RS32155
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,784,629 + BPHYT_RS32140 0.72 -0.7 2,784,630 - BPHYT_RS32140 0.72 -1.1 2,784,708 - BPHYT_RS32140 0.76 -0.9 2,785,140 + -1.3 2,785,140 + +1.8 2,785,140 + -0.5 2,785,141 - +0.7 2,785,141 - -1.0 2,785,328 - BPHYT_RS32145 0.11 -2.0 2,785,408 + BPHYT_RS32145 0.15 -0.2 2,785,409 - BPHYT_RS32145 0.15 -1.9 2,785,585 + BPHYT_RS32145 0.25 -0.7 2,785,826 - BPHYT_RS32145 0.39 -0.8 2,785,859 + BPHYT_RS32145 0.41 -1.8 2,785,859 + BPHYT_RS32145 0.41 -1.6 2,785,946 - BPHYT_RS32145 0.46 -1.1 2,786,135 - BPHYT_RS32145 0.57 -0.9 2,786,414 - BPHYT_RS32145 0.73 -1.0 2,786,581 - BPHYT_RS32145 0.82 -1.5 2,786,740 + +0.1 2,786,847 + -1.1 2,786,946 + BPHYT_RS32150 0.14 +0.5 2,787,006 - BPHYT_RS32150 0.28 +1.1 2,787,147 + BPHYT_RS32150 0.63 +0.2 2,787,710 - BPHYT_RS32155 0.36 +0.5 2,787,816 - BPHYT_RS32155 0.46 -1.1 2,787,816 - BPHYT_RS32155 0.46 +0.2
Or see this region's nucleotide sequence