Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32010

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32000 and BPHYT_RS32005 are separated by 98 nucleotidesBPHYT_RS32005 and BPHYT_RS32010 are separated by 100 nucleotidesBPHYT_RS32010 and BPHYT_RS32015 overlap by 4 nucleotides BPHYT_RS32000: BPHYT_RS32000 - integrase, at 2,739,569 to 2,741,005 _RS32000 BPHYT_RS32005: BPHYT_RS32005 - hypothetical protein, at 2,741,104 to 2,741,682 _RS32005 BPHYT_RS32010: BPHYT_RS32010 - DEAD/DEAH box helicase, at 2,741,783 to 2,744,374 _RS32010 BPHYT_RS32015: BPHYT_RS32015 - hypothetical protein, at 2,744,371 to 2,746,176 _RS32015 Position (kb) 2741 2742 2743 2744 2745Strain fitness (log2 ratio) -1 0 1 2 3at 2741.875 kb on + strandat 2741.875 kb on + strandat 2741.875 kb on + strandat 2741.876 kb on - strandat 2741.974 kb on + strandat 2741.975 kb on - strandat 2742.054 kb on - strand, within BPHYT_RS32010at 2742.064 kb on + strand, within BPHYT_RS32010at 2742.092 kb on + strand, within BPHYT_RS32010at 2742.092 kb on + strand, within BPHYT_RS32010at 2742.216 kb on - strand, within BPHYT_RS32010at 2742.266 kb on + strand, within BPHYT_RS32010at 2742.298 kb on - strand, within BPHYT_RS32010at 2743.007 kb on + strand, within BPHYT_RS32010at 2743.076 kb on - strand, within BPHYT_RS32010at 2743.169 kb on + strand, within BPHYT_RS32010at 2743.169 kb on + strand, within BPHYT_RS32010at 2743.596 kb on - strand, within BPHYT_RS32010at 2743.620 kb on - strand, within BPHYT_RS32010at 2743.817 kb on + strand, within BPHYT_RS32010at 2743.852 kb on + strand, within BPHYT_RS32010at 2743.853 kb on - strand, within BPHYT_RS32010at 2743.853 kb on - strand, within BPHYT_RS32010at 2743.853 kb on - strand, within BPHYT_RS32010at 2743.855 kb on - strand, within BPHYT_RS32010at 2743.928 kb on + strand, within BPHYT_RS32010at 2744.041 kb on + strand, within BPHYT_RS32010at 2744.121 kb on + strandat 2744.199 kb on + strandat 2744.200 kb on - strandat 2744.582 kb on - strand, within BPHYT_RS32015at 2744.784 kb on + strand, within BPHYT_RS32015at 2744.836 kb on - strand, within BPHYT_RS32015at 2745.022 kb on + strand, within BPHYT_RS32015at 2745.022 kb on + strand, within BPHYT_RS32015at 2745.109 kb on - strand, within BPHYT_RS32015at 2745.138 kb on - strand, within BPHYT_RS32015at 2745.180 kb on + strand, within BPHYT_RS32015at 2745.181 kb on - strand, within BPHYT_RS32015

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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2,741,875 + -0.6
2,741,875 + -0.7
2,741,875 + -1.1
2,741,876 - +3.0
2,741,974 + +0.1
2,741,975 - -0.7
2,742,054 - BPHYT_RS32010 0.10 +1.7
2,742,064 + BPHYT_RS32010 0.11 +0.8
2,742,092 + BPHYT_RS32010 0.12 -1.2
2,742,092 + BPHYT_RS32010 0.12 -0.7
2,742,216 - BPHYT_RS32010 0.17 -0.4
2,742,266 + BPHYT_RS32010 0.19 +0.9
2,742,298 - BPHYT_RS32010 0.20 +1.1
2,743,007 + BPHYT_RS32010 0.47 +0.1
2,743,076 - BPHYT_RS32010 0.50 +0.0
2,743,169 + BPHYT_RS32010 0.53 -0.2
2,743,169 + BPHYT_RS32010 0.53 +1.2
2,743,596 - BPHYT_RS32010 0.70 -0.2
2,743,620 - BPHYT_RS32010 0.71 -0.4
2,743,817 + BPHYT_RS32010 0.78 -0.1
2,743,852 + BPHYT_RS32010 0.80 -0.9
2,743,853 - BPHYT_RS32010 0.80 -0.9
2,743,853 - BPHYT_RS32010 0.80 +0.8
2,743,853 - BPHYT_RS32010 0.80 -0.5
2,743,855 - BPHYT_RS32010 0.80 -0.0
2,743,928 + BPHYT_RS32010 0.83 +0.6
2,744,041 + BPHYT_RS32010 0.87 -0.7
2,744,121 + +0.1
2,744,199 + +0.9
2,744,200 - +0.1
2,744,582 - BPHYT_RS32015 0.12 -0.7
2,744,784 + BPHYT_RS32015 0.23 +0.8
2,744,836 - BPHYT_RS32015 0.26 +0.0
2,745,022 + BPHYT_RS32015 0.36 -0.1
2,745,022 + BPHYT_RS32015 0.36 +0.6
2,745,109 - BPHYT_RS32015 0.41 -0.2
2,745,138 - BPHYT_RS32015 0.42 -1.5
2,745,180 + BPHYT_RS32015 0.45 -1.3
2,745,181 - BPHYT_RS32015 0.45 -0.5

Or see this region's nucleotide sequence