Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31670 and BPHYT_RS31675 are separated by 298 nucleotides BPHYT_RS31675 and BPHYT_RS31680 are separated by 129 nucleotides BPHYT_RS31680 and BPHYT_RS31685 are separated by 57 nucleotides
BPHYT_RS31670: BPHYT_RS31670 - transposase, at 2,661,861 to 2,662,196
_RS31670
BPHYT_RS31675: BPHYT_RS31675 - hypothetical protein, at 2,662,495 to 2,662,821
_RS31675
BPHYT_RS31680: BPHYT_RS31680 - dehydrogenase, at 2,662,951 to 2,663,844
_RS31680
BPHYT_RS31685: BPHYT_RS31685 - D-isomer specific 2-hydroxyacid dehydrogenase, at 2,663,902 to 2,664,882
_RS31685
Position (kb)
2662
2663
2664 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2662.153 kb on - strand, within BPHYT_RS31670 at 2662.227 kb on - strand at 2662.554 kb on + strand, within BPHYT_RS31675 at 2662.696 kb on - strand, within BPHYT_RS31675 at 2662.923 kb on - strand at 2662.938 kb on - strand at 2662.940 kb on + strand at 2662.944 kb on + strand at 2663.263 kb on - strand, within BPHYT_RS31680 at 2663.354 kb on + strand, within BPHYT_RS31680 at 2663.378 kb on + strand, within BPHYT_RS31680 at 2663.527 kb on - strand, within BPHYT_RS31680 at 2663.647 kb on - strand, within BPHYT_RS31680 at 2663.844 kb on + strand at 2664.285 kb on - strand, within BPHYT_RS31685 at 2664.307 kb on - strand, within BPHYT_RS31685 at 2664.582 kb on + strand, within BPHYT_RS31685
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,662,153 - BPHYT_RS31670 0.87 -0.5 2,662,227 - -0.2 2,662,554 + BPHYT_RS31675 0.18 -0.5 2,662,696 - BPHYT_RS31675 0.61 -0.2 2,662,923 - -0.8 2,662,938 - +0.6 2,662,940 + -1.1 2,662,944 + -0.2 2,663,263 - BPHYT_RS31680 0.35 -0.7 2,663,354 + BPHYT_RS31680 0.45 +0.8 2,663,378 + BPHYT_RS31680 0.48 +4.9 2,663,527 - BPHYT_RS31680 0.64 -0.6 2,663,647 - BPHYT_RS31680 0.78 -1.1 2,663,844 + +0.1 2,664,285 - BPHYT_RS31685 0.39 +2.2 2,664,307 - BPHYT_RS31685 0.41 +0.0 2,664,582 + BPHYT_RS31685 0.69 +1.0
Or see this region's nucleotide sequence