Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31595 and BPHYT_RS31600 are separated by 225 nucleotides BPHYT_RS31600 and BPHYT_RS31605 are separated by 54 nucleotides BPHYT_RS31605 and BPHYT_RS31610 are separated by 56 nucleotides
BPHYT_RS31595: BPHYT_RS31595 - hypothetical protein, at 2,639,982 to 2,640,674
_RS31595
BPHYT_RS31600: BPHYT_RS31600 - hypothetical protein, at 2,640,900 to 2,641,340
_RS31600
BPHYT_RS31605: BPHYT_RS31605 - glucoamylase, at 2,641,395 to 2,643,185
_RS31605
BPHYT_RS31610: BPHYT_RS31610 - short-chain dehydrogenase, at 2,643,242 to 2,644,240
_RS31610
Position (kb)
2641
2642
2643
2644 Strain fitness (log2 ratio)
-1
0
1 at 2641.075 kb on + strand, within BPHYT_RS31600 at 2641.075 kb on + strand, within BPHYT_RS31600 at 2641.817 kb on - strand, within BPHYT_RS31605 at 2641.971 kb on + strand, within BPHYT_RS31605 at 2642.272 kb on + strand, within BPHYT_RS31605 at 2642.273 kb on - strand, within BPHYT_RS31605 at 2642.884 kb on - strand, within BPHYT_RS31605 at 2643.100 kb on - strand at 2643.244 kb on - strand at 2643.452 kb on - strand, within BPHYT_RS31610 at 2643.452 kb on - strand, within BPHYT_RS31610
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,641,075 + BPHYT_RS31600 0.40 +0.2 2,641,075 + BPHYT_RS31600 0.40 -0.6 2,641,817 - BPHYT_RS31605 0.24 +0.7 2,641,971 + BPHYT_RS31605 0.32 +0.7 2,642,272 + BPHYT_RS31605 0.49 +0.4 2,642,273 - BPHYT_RS31605 0.49 +0.2 2,642,884 - BPHYT_RS31605 0.83 -0.6 2,643,100 - +0.8 2,643,244 - +0.9 2,643,452 - BPHYT_RS31610 0.21 +0.1 2,643,452 - BPHYT_RS31610 0.21 -0.7
Or see this region's nucleotide sequence