Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31290

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31285 and BPHYT_RS31290 are separated by 223 nucleotidesBPHYT_RS31290 and BPHYT_RS31295 overlap by 4 nucleotides BPHYT_RS31285: BPHYT_RS31285 - universal stress protein A, at 2,577,670 to 2,578,515 _RS31285 BPHYT_RS31290: BPHYT_RS31290 - histidine kinase, at 2,578,739 to 2,580,085 _RS31290 BPHYT_RS31295: BPHYT_RS31295 - LuxR family transcriptional regulator, at 2,580,082 to 2,580,732 _RS31295 Position (kb) 2578 2579 2580 2581Strain fitness (log2 ratio) -2 -1 0 1at 2577.814 kb on + strand, within BPHYT_RS31285at 2577.815 kb on - strand, within BPHYT_RS31285at 2577.838 kb on + strandat 2578.046 kb on - strand, within BPHYT_RS31285at 2578.046 kb on - strand, within BPHYT_RS31285at 2578.592 kb on - strandat 2578.938 kb on - strand, within BPHYT_RS31290at 2579.891 kb on - strand, within BPHYT_RS31290at 2580.139 kb on - strandat 2580.451 kb on + strand, within BPHYT_RS31295at 2580.451 kb on + strand, within BPHYT_RS31295at 2580.452 kb on - strand, within BPHYT_RS31295at 2580.452 kb on - strand, within BPHYT_RS31295at 2580.629 kb on - strand, within BPHYT_RS31295at 2580.629 kb on - strand, within BPHYT_RS31295at 2580.700 kb on - strandat 2580.815 kb on + strandat 2580.815 kb on + strandat 2580.933 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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2,577,814 + BPHYT_RS31285 0.17 +0.5
2,577,815 - BPHYT_RS31285 0.17 -1.0
2,577,838 + +0.9
2,578,046 - BPHYT_RS31285 0.44 -1.2
2,578,046 - BPHYT_RS31285 0.44 -0.6
2,578,592 - -0.8
2,578,938 - BPHYT_RS31290 0.15 -1.8
2,579,891 - BPHYT_RS31290 0.86 -0.3
2,580,139 - +0.6
2,580,451 + BPHYT_RS31295 0.57 +0.2
2,580,451 + BPHYT_RS31295 0.57 -1.4
2,580,452 - BPHYT_RS31295 0.57 -0.1
2,580,452 - BPHYT_RS31295 0.57 +0.4
2,580,629 - BPHYT_RS31295 0.84 +1.3
2,580,629 - BPHYT_RS31295 0.84 -0.0
2,580,700 - +0.5
2,580,815 + +0.4
2,580,815 + +1.7
2,580,933 - -1.4

Or see this region's nucleotide sequence