Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31170 and BPHYT_RS31175 are separated by 145 nucleotides BPHYT_RS31175 and BPHYT_RS31180 are separated by 6 nucleotides BPHYT_RS31180 and BPHYT_RS31185 are separated by 124 nucleotides
BPHYT_RS31170: BPHYT_RS31170 - hypothetical protein, at 2,550,548 to 2,552,365
_RS31170
BPHYT_RS31175: BPHYT_RS31175 - RNA polymerase sigma70 factor, at 2,552,511 to 2,553,785
_RS31175
BPHYT_RS31180: BPHYT_RS31180 - DGPFAETKE family protein, at 2,553,792 to 2,554,163
_RS31180
BPHYT_RS31185: BPHYT_RS31185 - LysR family transcriptional regulator, at 2,554,288 to 2,555,199
_RS31185
Position (kb)
2552
2553
2554 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2551.537 kb on - strand, within BPHYT_RS31170 at 2551.625 kb on - strand, within BPHYT_RS31170 at 2551.762 kb on + strand, within BPHYT_RS31170 at 2551.763 kb on - strand, within BPHYT_RS31170 at 2551.770 kb on + strand, within BPHYT_RS31170 at 2552.057 kb on - strand, within BPHYT_RS31170 at 2552.453 kb on + strand at 2552.453 kb on + strand at 2552.454 kb on - strand at 2552.454 kb on - strand at 2552.713 kb on + strand, within BPHYT_RS31175 at 2552.871 kb on + strand, within BPHYT_RS31175 at 2552.872 kb on - strand, within BPHYT_RS31175 at 2552.982 kb on - strand, within BPHYT_RS31175 at 2552.982 kb on - strand, within BPHYT_RS31175 at 2552.982 kb on - strand, within BPHYT_RS31175 at 2553.010 kb on + strand, within BPHYT_RS31175 at 2553.010 kb on + strand, within BPHYT_RS31175 at 2553.011 kb on - strand, within BPHYT_RS31175 at 2553.055 kb on + strand, within BPHYT_RS31175 at 2553.055 kb on + strand, within BPHYT_RS31175 at 2554.142 kb on - strand at 2554.157 kb on - strand at 2554.194 kb on - strand at 2554.198 kb on + strand at 2554.198 kb on + strand at 2554.199 kb on - strand at 2554.390 kb on + strand, within BPHYT_RS31185 at 2554.390 kb on + strand, within BPHYT_RS31185 at 2554.687 kb on + strand, within BPHYT_RS31185
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,551,537 - BPHYT_RS31170 0.54 +2.7 2,551,625 - BPHYT_RS31170 0.59 +0.6 2,551,762 + BPHYT_RS31170 0.67 -1.7 2,551,763 - BPHYT_RS31170 0.67 +1.6 2,551,770 + BPHYT_RS31170 0.67 -0.7 2,552,057 - BPHYT_RS31170 0.83 +0.4 2,552,453 + +0.1 2,552,453 + +1.2 2,552,454 - +0.2 2,552,454 - +0.2 2,552,713 + BPHYT_RS31175 0.16 +0.5 2,552,871 + BPHYT_RS31175 0.28 -0.1 2,552,872 - BPHYT_RS31175 0.28 +0.0 2,552,982 - BPHYT_RS31175 0.37 +1.3 2,552,982 - BPHYT_RS31175 0.37 -0.0 2,552,982 - BPHYT_RS31175 0.37 -0.1 2,553,010 + BPHYT_RS31175 0.39 -2.3 2,553,010 + BPHYT_RS31175 0.39 -2.3 2,553,011 - BPHYT_RS31175 0.39 +0.2 2,553,055 + BPHYT_RS31175 0.43 -0.3 2,553,055 + BPHYT_RS31175 0.43 -1.5 2,554,142 - +0.3 2,554,157 - -1.0 2,554,194 - +1.5 2,554,198 + +1.8 2,554,198 + -0.1 2,554,199 - -0.3 2,554,390 + BPHYT_RS31185 0.11 -1.9 2,554,390 + BPHYT_RS31185 0.11 +0.0 2,554,687 + BPHYT_RS31185 0.44 -0.3
Or see this region's nucleotide sequence