Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30855 and BPHYT_RS30860 are separated by 8 nucleotides BPHYT_RS30860 and BPHYT_RS30865 overlap by 1 nucleotides BPHYT_RS30865 and BPHYT_RS30870 are separated by 109 nucleotides
BPHYT_RS30855: BPHYT_RS30855 - sugar ABC transporter substrate-binding protein, at 2,477,576 to 2,478,664
_RS30855
BPHYT_RS30860: BPHYT_RS30860 - sugar ABC transporter permease, at 2,478,673 to 2,479,662
_RS30860
BPHYT_RS30865: BPHYT_RS30865 - sugar ABC transporter ATP-binding protein, at 2,479,662 to 2,480,432
_RS30865
BPHYT_RS30870: BPHYT_RS30870 - chromate transporter, at 2,480,542 to 2,481,078
_RS30870
Position (kb)
2478
2479
2480 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2477.834 kb on + strand, within BPHYT_RS30855 at 2477.834 kb on + strand, within BPHYT_RS30855 at 2478.062 kb on + strand, within BPHYT_RS30855 at 2478.063 kb on - strand, within BPHYT_RS30855 at 2478.949 kb on + strand, within BPHYT_RS30860 at 2479.263 kb on + strand, within BPHYT_RS30860 at 2479.960 kb on - strand at 2480.164 kb on + strand, within BPHYT_RS30865 at 2480.164 kb on + strand, within BPHYT_RS30865 at 2480.165 kb on - strand, within BPHYT_RS30865 at 2480.284 kb on - strand, within BPHYT_RS30865
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 2,477,834 + BPHYT_RS30855 0.24 +2.3 2,477,834 + BPHYT_RS30855 0.24 -0.6 2,478,062 + BPHYT_RS30855 0.45 +1.0 2,478,063 - BPHYT_RS30855 0.45 +0.2 2,478,949 + BPHYT_RS30860 0.28 -0.3 2,479,263 + BPHYT_RS30860 0.60 +0.3 2,479,960 - -0.6 2,480,164 + BPHYT_RS30865 0.65 +4.7 2,480,164 + BPHYT_RS30865 0.65 +3.0 2,480,165 - BPHYT_RS30865 0.65 -0.2 2,480,284 - BPHYT_RS30865 0.81 +1.8
Or see this region's nucleotide sequence