Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29875

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29870 and BPHYT_RS29875 are separated by 105 nucleotidesBPHYT_RS29875 and BPHYT_RS29880 are separated by 62 nucleotides BPHYT_RS29870: BPHYT_RS29870 - 2-dehydropantoate 2-reductase, at 2,254,041 to 2,255,018 _RS29870 BPHYT_RS29875: BPHYT_RS29875 - betaine-aldehyde dehydrogenase, at 2,255,124 to 2,256,575 _RS29875 BPHYT_RS29880: BPHYT_RS29880 - LysR family transcriptional regulator, at 2,256,638 to 2,257,609 _RS29880 Position (kb) 2255 2256 2257Strain fitness (log2 ratio) -1 0 1 2 3 4at 2254.629 kb on - strand, within BPHYT_RS29870at 2256.157 kb on + strand, within BPHYT_RS29875at 2256.158 kb on - strand, within BPHYT_RS29875at 2256.158 kb on - strand, within BPHYT_RS29875at 2256.220 kb on + strand, within BPHYT_RS29875at 2256.630 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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2,254,629 - BPHYT_RS29870 0.60 +0.1
2,256,157 + BPHYT_RS29875 0.71 +0.8
2,256,158 - BPHYT_RS29875 0.71 -0.1
2,256,158 - BPHYT_RS29875 0.71 +4.0
2,256,220 + BPHYT_RS29875 0.75 -1.0
2,256,630 - -0.3

Or see this region's nucleotide sequence