Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28725 and BPHYT_RS28730 are separated by 423 nucleotides BPHYT_RS28730 and BPHYT_RS28735 are separated by 160 nucleotides BPHYT_RS28735 and BPHYT_RS28740 are separated by 29 nucleotides
BPHYT_RS28725: BPHYT_RS28725 - oxidoreductase, at 1,997,596 to 1,998,615
_RS28725
BPHYT_RS28730: BPHYT_RS28730 - galactarate dehydratase, at 1,999,039 to 2,000,550
_RS28730
BPHYT_RS28735: BPHYT_RS28735 - hypothetical protein, at 2,000,711 to 2,001,406
_RS28735
BPHYT_RS28740: BPHYT_RS28740 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,001,436 to 2,002,326
_RS28740
Position (kb)
1999
2000
2001 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1998.061 kb on + strand, within BPHYT_RS28725 at 1998.132 kb on + strand, within BPHYT_RS28725 at 1998.352 kb on - strand, within BPHYT_RS28725 at 1998.352 kb on - strand, within BPHYT_RS28725 at 1998.356 kb on - strand, within BPHYT_RS28725 at 1998.451 kb on + strand, within BPHYT_RS28725 at 1998.451 kb on + strand, within BPHYT_RS28725 at 1998.452 kb on - strand, within BPHYT_RS28725 at 1998.725 kb on + strand at 1999.004 kb on + strand at 1999.040 kb on + strand at 1999.041 kb on - strand at 1999.066 kb on + strand at 1999.067 kb on - strand at 1999.616 kb on - strand, within BPHYT_RS28730 at 1999.719 kb on + strand, within BPHYT_RS28730 at 2000.202 kb on - strand, within BPHYT_RS28730 at 2000.341 kb on + strand, within BPHYT_RS28730 at 2000.342 kb on - strand, within BPHYT_RS28730 at 2000.817 kb on - strand, within BPHYT_RS28735 at 2000.878 kb on + strand, within BPHYT_RS28735 at 2000.879 kb on - strand, within BPHYT_RS28735 at 2001.073 kb on - strand, within BPHYT_RS28735 at 2001.077 kb on - strand, within BPHYT_RS28735 at 2001.077 kb on - strand, within BPHYT_RS28735 at 2001.406 kb on + strand at 2001.438 kb on - strand at 2001.480 kb on + strand at 2001.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,998,061 + BPHYT_RS28725 0.46 +0.2 1,998,132 + BPHYT_RS28725 0.53 +0.7 1,998,352 - BPHYT_RS28725 0.74 +0.4 1,998,352 - BPHYT_RS28725 0.74 -0.3 1,998,356 - BPHYT_RS28725 0.75 +1.3 1,998,451 + BPHYT_RS28725 0.84 -0.8 1,998,451 + BPHYT_RS28725 0.84 +2.4 1,998,452 - BPHYT_RS28725 0.84 +0.4 1,998,725 + +0.9 1,999,004 + +0.4 1,999,040 + -1.6 1,999,041 - +0.2 1,999,066 + +0.7 1,999,067 - -0.7 1,999,616 - BPHYT_RS28730 0.38 -2.3 1,999,719 + BPHYT_RS28730 0.45 -0.6 2,000,202 - BPHYT_RS28730 0.77 -0.3 2,000,341 + BPHYT_RS28730 0.86 +1.1 2,000,342 - BPHYT_RS28730 0.86 -0.8 2,000,817 - BPHYT_RS28735 0.15 +0.7 2,000,878 + BPHYT_RS28735 0.24 -0.2 2,000,879 - BPHYT_RS28735 0.24 +1.4 2,001,073 - BPHYT_RS28735 0.52 -0.8 2,001,077 - BPHYT_RS28735 0.53 +0.1 2,001,077 - BPHYT_RS28735 0.53 +0.3 2,001,406 + +0.6 2,001,438 - +0.8 2,001,480 + -0.1 2,001,489 - +3.5
Or see this region's nucleotide sequence