Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28730

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28725 and BPHYT_RS28730 are separated by 423 nucleotidesBPHYT_RS28730 and BPHYT_RS28735 are separated by 160 nucleotidesBPHYT_RS28735 and BPHYT_RS28740 are separated by 29 nucleotides BPHYT_RS28725: BPHYT_RS28725 - oxidoreductase, at 1,997,596 to 1,998,615 _RS28725 BPHYT_RS28730: BPHYT_RS28730 - galactarate dehydratase, at 1,999,039 to 2,000,550 _RS28730 BPHYT_RS28735: BPHYT_RS28735 - hypothetical protein, at 2,000,711 to 2,001,406 _RS28735 BPHYT_RS28740: BPHYT_RS28740 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,001,436 to 2,002,326 _RS28740 Position (kb) 1999 2000 2001Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1998.061 kb on + strand, within BPHYT_RS28725at 1998.132 kb on + strand, within BPHYT_RS28725at 1998.352 kb on - strand, within BPHYT_RS28725at 1998.352 kb on - strand, within BPHYT_RS28725at 1998.356 kb on - strand, within BPHYT_RS28725at 1998.451 kb on + strand, within BPHYT_RS28725at 1998.451 kb on + strand, within BPHYT_RS28725at 1998.452 kb on - strand, within BPHYT_RS28725at 1998.725 kb on + strandat 1999.004 kb on + strandat 1999.040 kb on + strandat 1999.041 kb on - strandat 1999.066 kb on + strandat 1999.067 kb on - strandat 1999.616 kb on - strand, within BPHYT_RS28730at 1999.719 kb on + strand, within BPHYT_RS28730at 2000.202 kb on - strand, within BPHYT_RS28730at 2000.341 kb on + strand, within BPHYT_RS28730at 2000.342 kb on - strand, within BPHYT_RS28730at 2000.817 kb on - strand, within BPHYT_RS28735at 2000.878 kb on + strand, within BPHYT_RS28735at 2000.879 kb on - strand, within BPHYT_RS28735at 2001.073 kb on - strand, within BPHYT_RS28735at 2001.077 kb on - strand, within BPHYT_RS28735at 2001.077 kb on - strand, within BPHYT_RS28735at 2001.406 kb on + strandat 2001.438 kb on - strandat 2001.480 kb on + strandat 2001.489 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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1,998,061 + BPHYT_RS28725 0.46 +0.2
1,998,132 + BPHYT_RS28725 0.53 +0.7
1,998,352 - BPHYT_RS28725 0.74 +0.4
1,998,352 - BPHYT_RS28725 0.74 -0.3
1,998,356 - BPHYT_RS28725 0.75 +1.3
1,998,451 + BPHYT_RS28725 0.84 -0.8
1,998,451 + BPHYT_RS28725 0.84 +2.4
1,998,452 - BPHYT_RS28725 0.84 +0.4
1,998,725 + +0.9
1,999,004 + +0.4
1,999,040 + -1.6
1,999,041 - +0.2
1,999,066 + +0.7
1,999,067 - -0.7
1,999,616 - BPHYT_RS28730 0.38 -2.3
1,999,719 + BPHYT_RS28730 0.45 -0.6
2,000,202 - BPHYT_RS28730 0.77 -0.3
2,000,341 + BPHYT_RS28730 0.86 +1.1
2,000,342 - BPHYT_RS28730 0.86 -0.8
2,000,817 - BPHYT_RS28735 0.15 +0.7
2,000,878 + BPHYT_RS28735 0.24 -0.2
2,000,879 - BPHYT_RS28735 0.24 +1.4
2,001,073 - BPHYT_RS28735 0.52 -0.8
2,001,077 - BPHYT_RS28735 0.53 +0.1
2,001,077 - BPHYT_RS28735 0.53 +0.3
2,001,406 + +0.6
2,001,438 - +0.8
2,001,480 + -0.1
2,001,489 - +3.5

Or see this region's nucleotide sequence