Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27780 and BPHYT_RS27785 are separated by 102 nucleotides BPHYT_RS27785 and BPHYT_RS27790 are separated by 85 nucleotides BPHYT_RS27790 and BPHYT_RS27795 are separated by 398 nucleotides
BPHYT_RS27780: BPHYT_RS27780 - acetyl-CoA synthetase, at 1,792,688 to 1,794,664
_RS27780
BPHYT_RS27785: BPHYT_RS27785 - hypothetical protein, at 1,794,767 to 1,795,372
_RS27785
BPHYT_RS27790: BPHYT_RS27790 - poly-beta-hydroxybutyrate polymerase, at 1,795,458 to 1,797,314
_RS27790
BPHYT_RS27795: BPHYT_RS27795 - nitroreductase, at 1,797,713 to 1,798,381
_RS27795
Position (kb)
1795
1796
1797
1798 Strain fitness (log2 ratio)
-2
-1
0
1 at 1795.253 kb on + strand, within BPHYT_RS27785 at 1795.359 kb on + strand at 1795.446 kb on + strand at 1795.447 kb on - strand at 1795.657 kb on + strand, within BPHYT_RS27790 at 1795.657 kb on + strand, within BPHYT_RS27790 at 1795.856 kb on - strand, within BPHYT_RS27790 at 1796.069 kb on - strand, within BPHYT_RS27790 at 1796.876 kb on - strand, within BPHYT_RS27790 at 1797.994 kb on + strand, within BPHYT_RS27795 at 1797.994 kb on + strand, within BPHYT_RS27795 at 1798.022 kb on + strand, within BPHYT_RS27795 at 1798.131 kb on - strand, within BPHYT_RS27795 at 1798.131 kb on - strand, within BPHYT_RS27795 at 1798.182 kb on + strand, within BPHYT_RS27795 at 1798.260 kb on + strand, within BPHYT_RS27795 at 1798.260 kb on + strand, within BPHYT_RS27795
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,795,253 + BPHYT_RS27785 0.80 -0.7 1,795,359 + -0.4 1,795,446 + +0.9 1,795,447 - -0.1 1,795,657 + BPHYT_RS27790 0.11 +0.1 1,795,657 + BPHYT_RS27790 0.11 -1.5 1,795,856 - BPHYT_RS27790 0.21 -0.9 1,796,069 - BPHYT_RS27790 0.33 -1.0 1,796,876 - BPHYT_RS27790 0.76 -2.1 1,797,994 + BPHYT_RS27795 0.42 -0.9 1,797,994 + BPHYT_RS27795 0.42 +0.2 1,798,022 + BPHYT_RS27795 0.46 -0.7 1,798,131 - BPHYT_RS27795 0.62 -0.9 1,798,131 - BPHYT_RS27795 0.62 -0.2 1,798,182 + BPHYT_RS27795 0.70 -0.3 1,798,260 + BPHYT_RS27795 0.82 -0.4 1,798,260 + BPHYT_RS27795 0.82 -0.7
Or see this region's nucleotide sequence