Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27490

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBPHYT_RS27485 and BPHYT_RS27490 are separated by 293 nucleotidesBPHYT_RS27490 and BPHYT_RS27495 are separated by 405 nucleotides BPHYT_RS27485: BPHYT_RS27485 - NAD-dependent dehydratase, at 1,742,626 to 1,743,279 _RS27485 BPHYT_RS27490: BPHYT_RS27490 - porin, at 1,743,573 to 1,744,748 _RS27490 BPHYT_RS27495: BPHYT_RS27495 - hypothetical protein, at 1,745,154 to 1,745,534 _RS27495 Position (kb) 1743 1744 1745Strain fitness (log2 ratio) -1 0 1 2 3 4at 1742.936 kb on - strand, within BPHYT_RS27485at 1743.109 kb on + strand, within BPHYT_RS27485at 1743.330 kb on + strandat 1743.438 kb on + strandat 1744.056 kb on + strand, within BPHYT_RS27490at 1744.057 kb on - strand, within BPHYT_RS27490at 1744.374 kb on + strand, within BPHYT_RS27490at 1744.492 kb on - strand, within BPHYT_RS27490at 1744.497 kb on + strand, within BPHYT_RS27490at 1745.087 kb on - strandat 1745.106 kb on + strandat 1745.140 kb on - strandat 1745.140 kb on - strandat 1745.140 kb on - strandat 1745.247 kb on + strand, within BPHYT_RS27495at 1745.556 kb on - strandat 1745.590 kb on - strandat 1745.717 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
remove
1,742,936 - BPHYT_RS27485 0.47 -0.4
1,743,109 + BPHYT_RS27485 0.74 +0.2
1,743,330 + +0.0
1,743,438 + +1.3
1,744,056 + BPHYT_RS27490 0.41 +0.0
1,744,057 - BPHYT_RS27490 0.41 -1.4
1,744,374 + BPHYT_RS27490 0.68 +4.4
1,744,492 - BPHYT_RS27490 0.78 -0.7
1,744,497 + BPHYT_RS27490 0.79 +0.2
1,745,087 - +0.2
1,745,106 + +0.5
1,745,140 - -1.1
1,745,140 - -0.9
1,745,140 - -0.4
1,745,247 + BPHYT_RS27495 0.24 +0.7
1,745,556 - -0.6
1,745,590 - -0.0
1,745,717 + +0.1

Or see this region's nucleotide sequence