Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27020 and BPHYT_RS27025 are separated by 119 nucleotides BPHYT_RS27025 and BPHYT_RS27030 are separated by 92 nucleotides BPHYT_RS27030 and BPHYT_RS27035 overlap by 4 nucleotides BPHYT_RS27035 and BPHYT_RS27040 are separated by 94 nucleotides
BPHYT_RS27020: BPHYT_RS27020 - hypothetical protein, at 1,640,093 to 1,640,335
_RS27020
BPHYT_RS27025: BPHYT_RS27025 - membrane protein, at 1,640,455 to 1,641,036
_RS27025
BPHYT_RS27030: BPHYT_RS27030 - transcriptional regulator, at 1,641,129 to 1,641,473
_RS27030
BPHYT_RS27035: BPHYT_RS27035 - membrane protein, at 1,641,470 to 1,642,033
_RS27035
BPHYT_RS27040: BPHYT_RS27040 - permease, at 1,642,128 to 1,642,679
_RS27040
Position (kb) 1641
1642 Strain fitness (log2 ratio) -1
0
1 at 1640.412 kb on + strand at 1641.010 kb on - strand at 1641.036 kb on + strand at 1641.192 kb on + strand, within BPHYT_RS27030 at 1642.013 kb on + strand at 1642.462 kb on + strand, within BPHYT_RS27040
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,640,412 + -0.7 1,641,010 - -0.6 1,641,036 + -0.2 1,641,192 + BPHYT_RS27030 0.18 +1.2 1,642,013 + +0.4 1,642,462 + BPHYT_RS27040 0.61 -1.4
Or see this region's nucleotide sequence