Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26695 and BPHYT_RS26700 are separated by 15 nucleotides BPHYT_RS26700 and BPHYT_RS26705 overlap by 4 nucleotides BPHYT_RS26705 and BPHYT_RS26710 are separated by 20 nucleotides BPHYT_RS26710 and BPHYT_RS26715 are separated by 266 nucleotides
BPHYT_RS26695: BPHYT_RS26695 - cobalamin biosynthesis protein CbiG, at 1,557,225 to 1,558,937
_RS26695
BPHYT_RS26700: BPHYT_RS26700 - hypothetical protein, at 1,558,953 to 1,559,564
_RS26700
BPHYT_RS26705: BPHYT_RS26705 - hypothetical protein, at 1,559,561 to 1,560,043
_RS26705
BPHYT_RS26710: BPHYT_RS26710 - Hcp1 family type VI secretion system effector, at 1,560,064 to 1,560,540
_RS26710
BPHYT_RS26715: BPHYT_RS26715 - GTP-binding protein, at 1,560,807 to 1,563,431
_RS26715
Position (kb) 1559
1560
1561 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 1558.671 kb on + strand, within BPHYT_RS26695 at 1558.672 kb on - strand, within BPHYT_RS26695 at 1558.806 kb on - strand at 1558.811 kb on - strand at 1558.998 kb on + strand at 1558.998 kb on + strand at 1559.027 kb on + strand, within BPHYT_RS26700 at 1559.056 kb on + strand, within BPHYT_RS26700 at 1559.064 kb on + strand, within BPHYT_RS26700 at 1559.145 kb on + strand, within BPHYT_RS26700 at 1559.159 kb on - strand, within BPHYT_RS26700 at 1559.183 kb on - strand, within BPHYT_RS26700 at 1559.199 kb on - strand, within BPHYT_RS26700 at 1559.492 kb on - strand, within BPHYT_RS26700 at 1559.680 kb on + strand, within BPHYT_RS26705 at 1559.681 kb on - strand, within BPHYT_RS26705 at 1559.881 kb on - strand, within BPHYT_RS26705 at 1559.883 kb on + strand, within BPHYT_RS26705 at 1559.884 kb on - strand, within BPHYT_RS26705 at 1560.034 kb on + strand at 1560.035 kb on - strand at 1560.044 kb on - strand at 1560.089 kb on + strand at 1560.089 kb on + strand at 1560.089 kb on + strand at 1560.090 kb on - strand at 1560.090 kb on - strand at 1560.090 kb on - strand at 1560.144 kb on - strand, within BPHYT_RS26710 at 1560.236 kb on + strand, within BPHYT_RS26710 at 1560.237 kb on - strand, within BPHYT_RS26710 at 1560.237 kb on - strand, within BPHYT_RS26710 at 1560.326 kb on + strand, within BPHYT_RS26710 at 1560.542 kb on - strand at 1560.622 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,558,671 + BPHYT_RS26695 0.84 -0.9 1,558,672 - BPHYT_RS26695 0.84 +8.3 1,558,806 - -0.1 1,558,811 - -0.9 1,558,998 + +0.6 1,558,998 + +1.4 1,559,027 + BPHYT_RS26700 0.12 +1.2 1,559,056 + BPHYT_RS26700 0.17 +0.3 1,559,064 + BPHYT_RS26700 0.18 -0.0 1,559,145 + BPHYT_RS26700 0.31 +0.8 1,559,159 - BPHYT_RS26700 0.34 +0.8 1,559,183 - BPHYT_RS26700 0.38 +5.0 1,559,199 - BPHYT_RS26700 0.40 -0.1 1,559,492 - BPHYT_RS26700 0.88 +0.9 1,559,680 + BPHYT_RS26705 0.25 -0.5 1,559,681 - BPHYT_RS26705 0.25 -1.5 1,559,881 - BPHYT_RS26705 0.66 -0.6 1,559,883 + BPHYT_RS26705 0.67 +0.3 1,559,884 - BPHYT_RS26705 0.67 -0.3 1,560,034 + +0.8 1,560,035 - -0.8 1,560,044 - -1.3 1,560,089 + +0.9 1,560,089 + +0.8 1,560,089 + -1.2 1,560,090 - +0.4 1,560,090 - -1.9 1,560,090 - +0.5 1,560,144 - BPHYT_RS26710 0.17 -0.1 1,560,236 + BPHYT_RS26710 0.36 -0.7 1,560,237 - BPHYT_RS26710 0.36 +2.0 1,560,237 - BPHYT_RS26710 0.36 +0.3 1,560,326 + BPHYT_RS26710 0.55 -0.2 1,560,542 - -0.5 1,560,622 + +1.5
Or see this region's nucleotide sequence