Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS26510

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS26495 and BPHYT_RS26500 are separated by 39 nucleotidesBPHYT_RS26500 and BPHYT_RS26505 overlap by 4 nucleotidesBPHYT_RS26505 and BPHYT_RS26510 are separated by 160 nucleotidesBPHYT_RS26510 and BPHYT_RS26515 are separated by 24 nucleotidesBPHYT_RS26515 and BPHYT_RS26520 overlap by 2 nucleotidesBPHYT_RS26520 and BPHYT_RS26525 are separated by 26 nucleotides BPHYT_RS26495: BPHYT_RS26495 - glutathione S-transferase, at 1,515,044 to 1,515,682 _RS26495 BPHYT_RS26500: BPHYT_RS26500 - ATPase, at 1,515,722 to 1,516,171 _RS26500 BPHYT_RS26505: BPHYT_RS26505 - ArsR family transcriptional regulator, at 1,516,168 to 1,516,503 _RS26505 BPHYT_RS26510: BPHYT_RS26510 - transcriptional regulator, at 1,516,664 to 1,517,569 _RS26510 BPHYT_RS26515: BPHYT_RS26515 - HAD family hydrolase, at 1,517,594 to 1,518,208 _RS26515 BPHYT_RS26520: BPHYT_RS26520 - hypothetical protein, at 1,518,207 to 1,518,386 _RS26520 BPHYT_RS26525: BPHYT_RS26525 - amino acid ABC transporter substrate-binding protein, at 1,518,413 to 1,519,564 _RS26525 Position (kb) 1516 1517 1518Strain fitness (log2 ratio) -2 -1 0 1 2at 1515.670 kb on - strandat 1516.857 kb on - strand, within BPHYT_RS26510at 1516.865 kb on + strand, within BPHYT_RS26510at 1517.175 kb on - strand, within BPHYT_RS26510at 1517.595 kb on + strandat 1517.595 kb on + strandat 1517.875 kb on + strand, within BPHYT_RS26515at 1517.875 kb on + strand, within BPHYT_RS26515

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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1,515,670 - -0.2
1,516,857 - BPHYT_RS26510 0.21 -1.9
1,516,865 + BPHYT_RS26510 0.22 -1.7
1,517,175 - BPHYT_RS26510 0.56 -0.0
1,517,595 + -0.9
1,517,595 + -0.5
1,517,875 + BPHYT_RS26515 0.46 +2.2
1,517,875 + BPHYT_RS26515 0.46 +0.5

Or see this region's nucleotide sequence