Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25940 and BPHYT_RS25945 are separated by 227 nucleotides BPHYT_RS25945 and BPHYT_RS25950 are separated by 122 nucleotides BPHYT_RS25950 and BPHYT_RS25955 overlap by 1 nucleotides BPHYT_RS25955 and BPHYT_RS25960 are separated by 136 nucleotides
BPHYT_RS25940: BPHYT_RS25940 - arylsulfate sulfotransferase, at 1,396,478 to 1,396,732
_RS25940
BPHYT_RS25945: BPHYT_RS25945 - hypothetical protein, at 1,396,960 to 1,397,223
_RS25945
BPHYT_RS25950: BPHYT_RS25950 - sensor histidine kinase, at 1,397,346 to 1,398,806
_RS25950
BPHYT_RS25955: BPHYT_RS25955 - transcriptional regulator, at 1,398,806 to 1,399,483
_RS25955
BPHYT_RS25960: BPHYT_RS25960 - ABC transporter, at 1,399,620 to 1,400,534
_RS25960
Position (kb)
1397
1398
1399 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1396.966 kb on + strand at 1397.199 kb on + strand at 1397.200 kb on - strand at 1397.241 kb on + strand at 1397.652 kb on - strand, within BPHYT_RS25950 at 1397.745 kb on + strand, within BPHYT_RS25950 at 1397.776 kb on + strand, within BPHYT_RS25950 at 1398.719 kb on - strand at 1398.719 kb on - strand at 1398.957 kb on + strand at 1398.958 kb on - strand, within BPHYT_RS25955 at 1399.622 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,396,966 + -0.5 1,397,199 + +0.2 1,397,200 - -1.0 1,397,241 + -0.8 1,397,652 - BPHYT_RS25950 0.21 -2.1 1,397,745 + BPHYT_RS25950 0.27 -1.3 1,397,776 + BPHYT_RS25950 0.29 -1.9 1,398,719 - +0.1 1,398,719 - -0.3 1,398,957 + +4.5 1,398,958 - BPHYT_RS25955 0.22 -0.8 1,399,622 - +0.2
Or see this region's nucleotide sequence