Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25140

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25130 and BPHYT_RS25135 are separated by 3 nucleotidesBPHYT_RS25135 and BPHYT_RS25140 overlap by 4 nucleotidesBPHYT_RS25140 and BPHYT_RS25145 are separated by 120 nucleotidesBPHYT_RS25145 and BPHYT_RS25150 are separated by 17 nucleotides BPHYT_RS25130: BPHYT_RS25130 - MFS transporter, at 1,210,793 to 1,212,211 _RS25130 BPHYT_RS25135: BPHYT_RS25135 - hypothetical protein, at 1,212,215 to 1,212,658 _RS25135 BPHYT_RS25140: BPHYT_RS25140 - hypothetical protein, at 1,212,655 to 1,213,164 _RS25140 BPHYT_RS25145: BPHYT_RS25145 - cupin, at 1,213,285 to 1,213,686 _RS25145 BPHYT_RS25150: BPHYT_RS25150 - class C beta-lactamase, at 1,213,704 to 1,214,870 _RS25150 Position (kb) 1212 1213 1214Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1211.822 kb on + strand, within BPHYT_RS25130at 1211.826 kb on + strand, within BPHYT_RS25130at 1212.020 kb on + strand, within BPHYT_RS25130at 1212.021 kb on - strand, within BPHYT_RS25130at 1212.216 kb on + strandat 1212.519 kb on + strand, within BPHYT_RS25135at 1212.629 kb on - strandat 1213.031 kb on - strand, within BPHYT_RS25140at 1213.082 kb on + strand, within BPHYT_RS25140at 1213.193 kb on + strandat 1213.240 kb on - strandat 1213.305 kb on + strandat 1213.305 kb on + strandat 1213.373 kb on - strand, within BPHYT_RS25145at 1213.396 kb on - strand, within BPHYT_RS25145at 1213.473 kb on - strand, within BPHYT_RS25145at 1213.740 kb on - strandat 1213.741 kb on + strandat 1214.137 kb on + strand, within BPHYT_RS25150

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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1,211,822 + BPHYT_RS25130 0.73 -0.0
1,211,826 + BPHYT_RS25130 0.73 -0.3
1,212,020 + BPHYT_RS25130 0.86 -0.4
1,212,021 - BPHYT_RS25130 0.87 -0.5
1,212,216 + +0.1
1,212,519 + BPHYT_RS25135 0.68 -0.8
1,212,629 - +0.2
1,213,031 - BPHYT_RS25140 0.74 +0.7
1,213,082 + BPHYT_RS25140 0.84 +3.4
1,213,193 + -0.9
1,213,240 - +0.3
1,213,305 + +3.2
1,213,305 + -0.2
1,213,373 - BPHYT_RS25145 0.22 -0.9
1,213,396 - BPHYT_RS25145 0.28 -0.8
1,213,473 - BPHYT_RS25145 0.47 -2.2
1,213,740 - -0.7
1,213,741 + +0.1
1,214,137 + BPHYT_RS25150 0.37 -0.2

Or see this region's nucleotide sequence