Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24805 and BPHYT_RS24810 are separated by 32 nucleotides BPHYT_RS24810 and BPHYT_RS24815 are separated by 12 nucleotides BPHYT_RS24815 and BPHYT_RS24820 overlap by 17 nucleotides
BPHYT_RS24805: BPHYT_RS24805 - sensor histidine kinase, at 1,132,611 to 1,135,100
_RS24805
BPHYT_RS24810: BPHYT_RS24810 - hypothetical protein, at 1,135,133 to 1,135,741
_RS24810
BPHYT_RS24815: BPHYT_RS24815 - cob(II)yrinic acid a,c-diamide reductase, at 1,135,754 to 1,136,413
_RS24815
BPHYT_RS24820: BPHYT_RS24820 - cobyrinic acid a,c-diamide synthase, at 1,136,397 to 1,137,695
_RS24820
Position (kb) 1135
1136
1137 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1134.760 kb on - strand, within BPHYT_RS24805 at 1134.760 kb on - strand, within BPHYT_RS24805 at 1135.104 kb on - strand at 1135.213 kb on + strand, within BPHYT_RS24810 at 1135.262 kb on + strand, within BPHYT_RS24810 at 1135.264 kb on + strand, within BPHYT_RS24810 at 1135.264 kb on + strand, within BPHYT_RS24810 at 1135.265 kb on - strand, within BPHYT_RS24810 at 1135.382 kb on + strand, within BPHYT_RS24810 at 1135.559 kb on + strand, within BPHYT_RS24810 at 1136.452 kb on + strand at 1136.452 kb on + strand at 1136.464 kb on + strand at 1136.723 kb on - strand, within BPHYT_RS24820 at 1136.723 kb on - strand, within BPHYT_RS24820 at 1136.919 kb on + strand, within BPHYT_RS24820 at 1137.075 kb on + strand, within BPHYT_RS24820 at 1137.075 kb on + strand, within BPHYT_RS24820
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,134,760 - BPHYT_RS24805 0.86 -0.7 1,134,760 - BPHYT_RS24805 0.86 -0.6 1,135,104 - +1.1 1,135,213 + BPHYT_RS24810 0.13 -0.3 1,135,262 + BPHYT_RS24810 0.21 -0.9 1,135,264 + BPHYT_RS24810 0.22 +0.1 1,135,264 + BPHYT_RS24810 0.22 -1.0 1,135,265 - BPHYT_RS24810 0.22 -2.0 1,135,382 + BPHYT_RS24810 0.41 +0.1 1,135,559 + BPHYT_RS24810 0.70 +3.0 1,136,452 + -2.6 1,136,452 + +1.1 1,136,464 + +1.3 1,136,723 - BPHYT_RS24820 0.25 -1.4 1,136,723 - BPHYT_RS24820 0.25 -0.6 1,136,919 + BPHYT_RS24820 0.40 +0.3 1,137,075 + BPHYT_RS24820 0.52 +0.1 1,137,075 + BPHYT_RS24820 0.52 +0.0
Or see this region's nucleotide sequence