Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24665 and BPHYT_RS24670 overlap by 4 nucleotides BPHYT_RS24670 and BPHYT_RS24675 overlap by 4 nucleotides BPHYT_RS24675 and BPHYT_RS24680 are separated by 11 nucleotides BPHYT_RS24680 and BPHYT_RS24685 are separated by 242 nucleotides
BPHYT_RS24665: BPHYT_RS24665 - ABC transporter permease, at 1,103,491 to 1,104,378
_RS24665
BPHYT_RS24670: BPHYT_RS24670 - ABC transporter permease, at 1,104,375 to 1,105,334
_RS24670
BPHYT_RS24675: BPHYT_RS24675 - spermidine/putrescine ABC transporter substrate-binding protein, at 1,105,331 to 1,106,425
_RS24675
BPHYT_RS24680: BPHYT_RS24680 - hypothetical protein, at 1,106,437 to 1,106,712
_RS24680
BPHYT_RS24685: BPHYT_RS24685 - LysR family transcriptional regulator, at 1,106,955 to 1,107,848
_RS24685
Position (kb) 1105
1106
1107 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1104.615 kb on + strand, within BPHYT_RS24670 at 1104.796 kb on + strand, within BPHYT_RS24670 at 1105.353 kb on + strand at 1105.635 kb on + strand, within BPHYT_RS24675 at 1105.636 kb on - strand, within BPHYT_RS24675 at 1105.636 kb on - strand, within BPHYT_RS24675 at 1105.696 kb on - strand, within BPHYT_RS24675 at 1105.932 kb on + strand, within BPHYT_RS24675 at 1105.968 kb on + strand, within BPHYT_RS24675 at 1105.969 kb on - strand, within BPHYT_RS24675 at 1105.969 kb on - strand, within BPHYT_RS24675 at 1106.249 kb on - strand, within BPHYT_RS24675 at 1106.490 kb on - strand, within BPHYT_RS24680 at 1106.529 kb on + strand, within BPHYT_RS24680 at 1106.529 kb on + strand, within BPHYT_RS24680 at 1106.530 kb on - strand, within BPHYT_RS24680 at 1106.582 kb on + strand, within BPHYT_RS24680 at 1106.816 kb on - strand at 1107.040 kb on + strand at 1107.093 kb on + strand, within BPHYT_RS24685 at 1107.094 kb on - strand, within BPHYT_RS24685 at 1107.384 kb on + strand, within BPHYT_RS24685 at 1107.385 kb on - strand, within BPHYT_RS24685
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,104,615 + BPHYT_RS24670 0.25 +0.1 1,104,796 + BPHYT_RS24670 0.44 +0.6 1,105,353 + -0.2 1,105,635 + BPHYT_RS24675 0.28 -0.3 1,105,636 - BPHYT_RS24675 0.28 -1.5 1,105,636 - BPHYT_RS24675 0.28 -0.2 1,105,696 - BPHYT_RS24675 0.33 -1.3 1,105,932 + BPHYT_RS24675 0.55 +1.2 1,105,968 + BPHYT_RS24675 0.58 -1.0 1,105,969 - BPHYT_RS24675 0.58 +0.3 1,105,969 - BPHYT_RS24675 0.58 -0.6 1,106,249 - BPHYT_RS24675 0.84 -0.1 1,106,490 - BPHYT_RS24680 0.19 -3.8 1,106,529 + BPHYT_RS24680 0.33 -0.8 1,106,529 + BPHYT_RS24680 0.33 -0.6 1,106,530 - BPHYT_RS24680 0.34 +0.6 1,106,582 + BPHYT_RS24680 0.53 -0.4 1,106,816 - +0.3 1,107,040 + -0.4 1,107,093 + BPHYT_RS24685 0.15 -0.4 1,107,094 - BPHYT_RS24685 0.16 -1.1 1,107,384 + BPHYT_RS24685 0.48 -1.5 1,107,385 - BPHYT_RS24685 0.48 -1.2
Or see this region's nucleotide sequence