Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24435 and BPHYT_RS24440 are separated by 4 nucleotides BPHYT_RS24440 and BPHYT_RS24445 overlap by 4 nucleotides BPHYT_RS24445 and BPHYT_RS24450 are separated by 259 nucleotides
BPHYT_RS24435: BPHYT_RS24435 - cell envelope biogenesis protein OmpA, at 1,048,325 to 1,049,287
_RS24435
BPHYT_RS24440: BPHYT_RS24440 - type VI secretion protein, at 1,049,292 to 1,050,284
_RS24440
BPHYT_RS24445: BPHYT_RS24445 - type VI secretion protein VasK, at 1,050,281 to 1,054,147
_RS24445
BPHYT_RS24450: BPHYT_RS24450 - D-alanyl-D-alanine carboxypeptidase, at 1,054,407 to 1,055,252
_RS24450
Position (kb)
1050
1051
1052
1053
1054
1055 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1049.450 kb on - strand, within BPHYT_RS24440 at 1049.807 kb on + strand, within BPHYT_RS24440 at 1050.054 kb on - strand, within BPHYT_RS24440 at 1050.054 kb on - strand, within BPHYT_RS24440 at 1051.105 kb on - strand, within BPHYT_RS24445 at 1051.385 kb on - strand, within BPHYT_RS24445 at 1051.386 kb on + strand, within BPHYT_RS24445 at 1051.387 kb on - strand, within BPHYT_RS24445 at 1051.624 kb on - strand, within BPHYT_RS24445 at 1051.759 kb on - strand, within BPHYT_RS24445 at 1051.844 kb on - strand, within BPHYT_RS24445 at 1051.844 kb on - strand, within BPHYT_RS24445 at 1051.905 kb on + strand, within BPHYT_RS24445 at 1052.163 kb on + strand, within BPHYT_RS24445 at 1052.164 kb on - strand, within BPHYT_RS24445 at 1052.310 kb on + strand, within BPHYT_RS24445 at 1052.311 kb on - strand, within BPHYT_RS24445 at 1052.328 kb on + strand, within BPHYT_RS24445 at 1052.329 kb on - strand, within BPHYT_RS24445 at 1052.364 kb on + strand, within BPHYT_RS24445 at 1052.365 kb on - strand, within BPHYT_RS24445 at 1052.542 kb on - strand, within BPHYT_RS24445 at 1052.542 kb on - strand, within BPHYT_RS24445 at 1052.823 kb on + strand, within BPHYT_RS24445 at 1052.936 kb on - strand, within BPHYT_RS24445 at 1053.036 kb on + strand, within BPHYT_RS24445 at 1053.217 kb on - strand, within BPHYT_RS24445 at 1053.306 kb on + strand, within BPHYT_RS24445 at 1053.378 kb on + strand, within BPHYT_RS24445 at 1053.379 kb on - strand, within BPHYT_RS24445 at 1053.592 kb on - strand, within BPHYT_RS24445 at 1053.646 kb on + strand, within BPHYT_RS24445 at 1053.647 kb on - strand, within BPHYT_RS24445 at 1054.055 kb on - strand at 1054.070 kb on + strand at 1054.071 kb on - strand at 1054.147 kb on + strand at 1054.225 kb on + strand at 1054.382 kb on - strand at 1054.425 kb on + strand at 1054.425 kb on + strand at 1054.521 kb on + strand, within BPHYT_RS24450 at 1054.522 kb on - strand, within BPHYT_RS24450 at 1054.529 kb on - strand, within BPHYT_RS24450 at 1054.609 kb on + strand, within BPHYT_RS24450 at 1054.619 kb on - strand, within BPHYT_RS24450 at 1054.744 kb on + strand, within BPHYT_RS24450 at 1054.849 kb on - strand, within BPHYT_RS24450 at 1054.998 kb on - strand, within BPHYT_RS24450 at 1055.114 kb on + strand, within BPHYT_RS24450 at 1055.133 kb on - strand, within BPHYT_RS24450
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 1,049,450 - BPHYT_RS24440 0.16 -0.0 1,049,807 + BPHYT_RS24440 0.52 -0.5 1,050,054 - BPHYT_RS24440 0.77 +0.5 1,050,054 - BPHYT_RS24440 0.77 -0.5 1,051,105 - BPHYT_RS24445 0.21 +0.2 1,051,385 - BPHYT_RS24445 0.29 +2.2 1,051,386 + BPHYT_RS24445 0.29 +0.4 1,051,387 - BPHYT_RS24445 0.29 +0.3 1,051,624 - BPHYT_RS24445 0.35 +0.6 1,051,759 - BPHYT_RS24445 0.38 +1.2 1,051,844 - BPHYT_RS24445 0.40 -0.0 1,051,844 - BPHYT_RS24445 0.40 +0.7 1,051,905 + BPHYT_RS24445 0.42 -2.9 1,052,163 + BPHYT_RS24445 0.49 +0.6 1,052,164 - BPHYT_RS24445 0.49 -0.1 1,052,310 + BPHYT_RS24445 0.52 +0.4 1,052,311 - BPHYT_RS24445 0.52 +1.4 1,052,328 + BPHYT_RS24445 0.53 -0.9 1,052,329 - BPHYT_RS24445 0.53 -1.1 1,052,364 + BPHYT_RS24445 0.54 -0.7 1,052,365 - BPHYT_RS24445 0.54 -0.2 1,052,542 - BPHYT_RS24445 0.58 +0.6 1,052,542 - BPHYT_RS24445 0.58 +0.6 1,052,823 + BPHYT_RS24445 0.66 +1.0 1,052,936 - BPHYT_RS24445 0.69 +0.2 1,053,036 + BPHYT_RS24445 0.71 +1.0 1,053,217 - BPHYT_RS24445 0.76 -0.3 1,053,306 + BPHYT_RS24445 0.78 +2.2 1,053,378 + BPHYT_RS24445 0.80 +0.7 1,053,379 - BPHYT_RS24445 0.80 -1.5 1,053,592 - BPHYT_RS24445 0.86 -1.2 1,053,646 + BPHYT_RS24445 0.87 -0.1 1,053,647 - BPHYT_RS24445 0.87 +0.1 1,054,055 - +0.3 1,054,070 + +0.5 1,054,071 - +0.7 1,054,147 + -1.4 1,054,225 + +1.1 1,054,382 - -1.9 1,054,425 + +0.4 1,054,425 + -0.1 1,054,521 + BPHYT_RS24450 0.13 +0.7 1,054,522 - BPHYT_RS24450 0.14 +1.1 1,054,529 - BPHYT_RS24450 0.14 +0.3 1,054,609 + BPHYT_RS24450 0.24 +0.3 1,054,619 - BPHYT_RS24450 0.25 +3.6 1,054,744 + BPHYT_RS24450 0.40 +2.0 1,054,849 - BPHYT_RS24450 0.52 +0.5 1,054,998 - BPHYT_RS24450 0.70 -0.9 1,055,114 + BPHYT_RS24450 0.84 -0.2 1,055,133 - BPHYT_RS24450 0.86 +0.4
Or see this region's nucleotide sequence