Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24175

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24170 and BPHYT_RS24175 are separated by 587 nucleotidesBPHYT_RS24175 and BPHYT_RS24180 are separated by 153 nucleotidesBPHYT_RS24180 and BPHYT_RS24185 are separated by 7 nucleotides BPHYT_RS24170: BPHYT_RS24170 - gluconolactonase, at 990,808 to 991,737 _RS24170 BPHYT_RS24175: BPHYT_RS24175 - C4-dicarboxylate ABC transporter, at 992,325 to 993,317 _RS24175 BPHYT_RS24180: BPHYT_RS24180 - C4-dicarboxylate ABC transporter substrate-binding protein, at 993,471 to 993,950 _RS24180 BPHYT_RS24185: BPHYT_RS24185 - C4-dicarboxylate ABC transporter permease, at 993,958 to 995,310 _RS24185 Position (kb) 992 993 994Strain fitness (log2 ratio) -1 0 1 2 3at 991.342 kb on + strand, within BPHYT_RS24170at 991.343 kb on - strand, within BPHYT_RS24170at 991.343 kb on - strand, within BPHYT_RS24170at 991.994 kb on - strandat 992.040 kb on + strandat 992.330 kb on + strandat 992.331 kb on - strandat 992.446 kb on - strand, within BPHYT_RS24175at 992.531 kb on + strand, within BPHYT_RS24175at 992.784 kb on + strand, within BPHYT_RS24175at 992.917 kb on - strand, within BPHYT_RS24175

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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991,342 + BPHYT_RS24170 0.57 -0.1
991,343 - BPHYT_RS24170 0.58 +0.9
991,343 - BPHYT_RS24170 0.58 -0.3
991,994 - -0.3
992,040 + +1.1
992,330 + -0.6
992,331 - +1.6
992,446 - BPHYT_RS24175 0.12 +2.8
992,531 + BPHYT_RS24175 0.21 +0.4
992,784 + BPHYT_RS24175 0.46 +0.1
992,917 - BPHYT_RS24175 0.60 +1.3

Or see this region's nucleotide sequence