Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23930

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23920 and BPHYT_RS23925 overlap by 4 nucleotidesBPHYT_RS23925 and BPHYT_RS23930 overlap by 13 nucleotidesBPHYT_RS23930 and BPHYT_RS23935 are separated by 2 nucleotides BPHYT_RS23920: BPHYT_RS23920 - malonate decarboxylase subunit beta, at 932,214 to 933,098 _RS23920 BPHYT_RS23925: BPHYT_RS23925 - malonate decarboxylase subunit delta, at 933,095 to 933,409 _RS23925 BPHYT_RS23930: BPHYT_RS23930 - triphosphoribosyl-dephospho-CoA synthase, at 933,397 to 934,320 _RS23930 BPHYT_RS23935: BPHYT_RS23935 - malonate decarboxylase subunit alpha, at 934,323 to 936,005 _RS23935 Position (kb) 933 934 935Strain fitness (log2 ratio) -3 -2 -1 0 1at 932.572 kb on + strand, within BPHYT_RS23920at 932.573 kb on - strand, within BPHYT_RS23920at 933.093 kb on - strandat 934.128 kb on + strand, within BPHYT_RS23930at 934.128 kb on + strand, within BPHYT_RS23930at 934.355 kb on - strandat 934.355 kb on - strandat 934.503 kb on + strand, within BPHYT_RS23935at 935.162 kb on - strand, within BPHYT_RS23935at 935.223 kb on + strand, within BPHYT_RS23935at 935.224 kb on - strand, within BPHYT_RS23935

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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932,572 + BPHYT_RS23920 0.40 -1.0
932,573 - BPHYT_RS23920 0.41 -0.6
933,093 - -2.0
934,128 + BPHYT_RS23930 0.79 -1.5
934,128 + BPHYT_RS23930 0.79 -3.0
934,355 - -1.8
934,355 - -1.5
934,503 + BPHYT_RS23935 0.11 +0.9
935,162 - BPHYT_RS23935 0.50 +1.5
935,223 + BPHYT_RS23935 0.53 -1.0
935,224 - BPHYT_RS23935 0.54 -0.6

Or see this region's nucleotide sequence