Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23525 and BPHYT_RS23530 are separated by 512 nucleotides BPHYT_RS23530 and BPHYT_RS23535 are separated by 190 nucleotides BPHYT_RS23535 and BPHYT_RS23540 are separated by 32 nucleotides
BPHYT_RS23525: BPHYT_RS23525 - hypothetical protein, at 845,703 to 846,050
_RS23525
BPHYT_RS23530: BPHYT_RS23530 - hypothetical protein, at 846,563 to 846,790
_RS23530
BPHYT_RS23535: BPHYT_RS23535 - ABC transporter substrate-binding protein, at 846,981 to 847,700
_RS23535
BPHYT_RS23540: BPHYT_RS23540 - RND transporter, at 847,733 to 849,241
_RS23540
Position (kb) 846
847
848 Strain fitness (log2 ratio) -2
-1
0
1
2 at 846.007 kb on - strand, within BPHYT_RS23525 at 846.342 kb on + strand at 846.414 kb on + strand at 846.453 kb on - strand at 846.487 kb on + strand at 846.877 kb on + strand at 846.882 kb on - strand at 847.433 kb on - strand, within BPHYT_RS23535 at 847.433 kb on - strand, within BPHYT_RS23535 at 848.175 kb on + strand, within BPHYT_RS23540 at 848.175 kb on + strand, within BPHYT_RS23540 at 848.176 kb on - strand, within BPHYT_RS23540
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 846,007 - BPHYT_RS23525 0.87 -1.0 846,342 + +0.9 846,414 + +1.2 846,453 - -0.2 846,487 + +0.5 846,877 + +0.4 846,882 - -0.4 847,433 - BPHYT_RS23535 0.63 -0.2 847,433 - BPHYT_RS23535 0.63 +2.4 848,175 + BPHYT_RS23540 0.29 -0.4 848,175 + BPHYT_RS23540 0.29 +1.2 848,176 - BPHYT_RS23540 0.29 -2.2
Or see this region's nucleotide sequence